Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1991-06-07
1994-10-11
Benzion, Gary
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
800DIG48, 4351721, 4352404, 43524045, 43524049, A01H 500, A01H 400, A01H 100
Patent
active
053549430
ABSTRACT:
Methods of in vitro culturing of tree tissue are disclosed in which high frequency tissue regeneration is achieved thereby increasing the likelihood of regenerating somaclonal variants having desirable traits. These methods include: culturing with a ratio of naphthaleneacetic acid and benzyladenine, or of benzyladenine and zeatin, in a tissue regeneration medium, the ratio being selected to induce a high frequency of tissue regeneration; culturing on a tissue regeneration medium having a high auxin concentration with a relatively low cytokinin concentration for a short period of time, e.g. up to ten days, to regenerate a high number of morphogenic callus cells; and, regenerating somatic embyros using a medium comprising 2,4-dichlorophenoxyacetic acid, benzyladenine, sucrose and glutamine, for high frequency regeneration of emboyogenic calli which are then developed into somatic embryos. These high frequency tissue regeneration methods are used to develop plants having tolerance to toxic chemicals by combining the methods with in vitro exposure to toxic chemical thereby to challenge somaclonal variants with the toxic chemical to test for and recover tolerant variants.
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Haissig Bruce E.
Michler Charles H.
Benzion Gary
Fado John D.
Silverstein M. Howard
The United States of America as represented by the Secretary of
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