Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-06-07
1998-11-10
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 2431, 935 77, 935 78, C12Q 168, C07H 2102, C07H 2104
Patent
active
058341935
ABSTRACT:
Methods and compositions for the measurement of telomere length have application in medical diagnostic, prognostic, and therapeutic procedures. The methods for measuring telomere length include primer extension-based methods and probe-based methods. The primer extension methods involve elongation of telomeric, linker, and/or subtelomeric based primers under conditions such that the telomere serves as a template for primer extension and that the resultant primer extension products can be compared to standards of known length to provide a measure of telomere length. The probe based methods allow for telomere length measurements using DNA from lysed or whole cells and involve hybridizing an excess of probe to all telomeric repeat sequences in the telomere, measuring the amount of bound probe, and correlating the amount of bound probe measured with telomere length.
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Allsopp Richard
Kim Nam Woo
Kozlowski Michael R.
Prowse Karen R.
Wang Sy-Shi
Geron Corporation
Kaster Kevin R.
Stracker Elaine C.
Zitomer Stephanie W.
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