Methods for determining pre-amplification levels of a nucleic ac

Details Methods for determining pre-amplification levels of a nucleic ac Methods for determining pre-amplification levels of a nucleic ac

1995-06-07

1998-01-20

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

Using fungi

435 6, 435 912, 435 9121, 435 913, 536221, 536231, 536 243, 536 2532, C12Q 168, C12P 1934, C07H 1900, C07H 2102

Patent

active

057100292

ABSTRACT:
Methods and kits for relating initial amounts of target nucleic acids present in a sample to target-specific amplification products. It has been discovered that the transcription-mediated amplification system is capable of producing a quantitative relationship between target input and target-specific output. Further, the present invention relates to methods for carefully controlling this relationship resulting in an unexpectedly high degree of reproducability. Also described are useful methods for extending the dynamic range of transcription-based amplification systems.

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