Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1999-01-27
2000-06-20
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 72, 435243, 4352521, 435822, 435960, 435975, 424 931, 424 934, 4242821, 424802, A61K 4500, C12N 100, C12N 112, G01N 3353
Patent
active
060776781
ABSTRACT:
This invention relates generally to in vitro methods for inducing or enhancing expression of enteric bacterial antigens and/or virulence factors thereby producing antigenically enhanced enteric bacteria, to methods for using antigenically enhanced enteric bacteria and to vaccines comprising antigenically enhanced enteric bacteria. A Campylobacter bacterium having enhanced antigenic property is disclosed. A culture medium useful for culturing the Campylobacteria comprises 0.05% to 3% bile or 0.025% to 0.6% of one or more bile acids or salts. In addition a divalent cation chelator can also be included in the culture medium. The bacteria culture is in a growth phase at early log phase and between early log phase and stationary phase. The enhanced antigenic property is a higher level of an immunogenic antigen when compared to the antigenic property of the same bacteria grown on brain heart infusion broth. Also, antibodies to Campylobacter bacteria or Campylobacteria in samples are detected by immunoassays.
REFERENCES:
patent: 4923801 (1990-05-01), Marshall et al.
patent: 5262156 (1993-11-01), Alemohammad
patent: 5403924 (1995-04-01), Cover et al.
patent: 5434253 (1995-07-01), Thompson et al.
patent: 5459041 (1995-10-01), Blaser
patent: 5527678 (1996-06-01), Blaser et al.
patent: 5538729 (1996-07-01), Czinn et al.
patent: 5610060 (1997-03-01), Ward et al.
patent: 5681736 (1997-10-01), Pace et al.
Andrews & Maurelli, 1992, mxiA of Shigella flexneri 2a, which facilitates export of invasion plasmid antigens, encodes a homolog of the low-calcium-response protein, LcrD, of Yersinia pestis. Infec. Immun. 60:3287-3295.
Baqar et al., 1995, "Immunogenicity and protective efficacy of a prototype Campylobacter killed whole-cell vaccine in mice." Infect. Immun. 63:3731-3735.
Bell & Manning, 1990, "A domestic ferret model of immunity to Campylobacter jejuni-induced enteric disease." Infect. Immun. 58:1848-1852.
Caldwell et al., 1983, "Simple adult rabbit model for Campylobacter jejuni enteritis." Infect. Immun. 42:1176-1182.
Chen et al., 1992, "Immunization against gastric helicobacter infection in a mouse/Heliocobacter felis model." Lancet 339:1120-1121.
Clements et al., "Adjuvant activity of Escherichia coli heat-labile enterotoxin and effect on the induction of oral tolerance in mice to unrelated protein antigens," Vaccine, 6:269-275, Jun. 1988.
Czinn et al., "Oral Immunization Protects Germ-Free Mice Against Infection From Helicobacter Felis," Gut, vol. 102(4), Part 2 Suppl., Apr. 1992.
Czinn et al., "Oral Immunization Against Helicobacter pylori," Infection and Immunity, 59:2359-2363, 1991.
Czinn et al., "Protection of germ-free mice after infection by Helicobacter felis after active oral or passive IgA immunization," Vaccine 11:637-642, 1993.
Czinn and Nedrud, 1991, Infection and Immunity 59:2359-2363.
Doig et al., "Production of a Conserved Adhesin by the Human Gastroduodenal Pathogen Helicobacter pylori," Journal of Bacteriology, 174:2539-2547, Apr. 1992.
Durham et al., 1994, 94th Am. Soc. Microbiol. Abstract P-36, p. 386.
Ferrero et al., The Importance of Urease in Acid Protection for the Gastric-colonising Bacteria Helicobacter pylori and Helicobacter felis sp. nov., Microbial Ecology in Health and Disease, 4:121-134, 1991.
Field et al., 1993, "Characteristics of an avirulent Campylobacter jejuni strain and its virulence-enhanced variants." J. Med. Microbiol. 38:293-300.
Gilmour et al., 1991, "Vaccine containing iron-regulated proteins of Pasteurella haemolytica A2 enhances protection against experimental pasteurellosis in lambs." Vaccine 9:137-140.
Grant et al., 1993, "Role of flagella in adherence, internalization, and translocation of Campylobacter jejuni in nonpolarized and polarized epithelial cell cultures." Infect. Immun. 61:1764-1771.
Hale et al., 1985, "Identification and antigenic characterization of virulence-associated, plasmid-coded proteins of Shigella spp. and enteroinvasive Escherichia coli." Infect. Immun. 50:620-629.
Heap et al., Immunisation and Gastric Colonisation with Helicobacter Felis, VIth International Workshop on Campylobacter, Hexicobacter and Related Organisms, Sydney, New South Wales, Australia, Oct. 7-10, 1991, Microb. Ecol. Health Dis. 4, 1991.
High et al., 1992, "IpaB of Shigella flexneri causes entry into epithelial cells and escape from the phagocytic vacuole." EMBO J. 11:1991-1999.
Konkel et al., 1993, J. Infect. Disc. 168:948-954.
Levenson et al., 1988, "Parental immunization with Shigella ribosomal vaccine elicits local Ig A response and primes for mucosal memory." Arch. Allergy Appl. Immunol. 87:25-31.
J. Mekalanos, 1992, J. Bacteriol. 174:1-7.
Mills et al., "Lipopolysaccharide Antigens of Helicobacter Pylori," VIth International Workshop on Campylobacter Helicobacter and Related Organisms, Sydney, New South Wales, Australia, Oct. 7-10, 1991, Microb. Ecol. Health Dis. 4, 1991.
Panigrahi et al., 1992, Infect. Immun. 60:4938-4944.
Pappo et al., "Effect of Oral Immunization with Recombinant Urease on Murine Helicobacter felis Gastritis," Infection and Immunity, 53:1246-1252, Apr. 1995.
Pavlovskis et al., 1992, "Significance of flagella in colonization resistance of rabbits immunized with Campylobacter spp." Infect. Immun. 59:2259-2264.
Pavlovskis et al., "Adjuvant Effect of Escherichia coli Heat-Labile Enterotoxin on Host Immune Response Following Vaccination with Non-Viable Campylobacter Antigens," VIth International Workshop on Campylobacter Helicobacte rand Related Organisms, Sydney, New South Wales, Australia, Oct. 7-10, 1991, Microb Ecol. Health Dis. 4, 1991.
S.M. Payne, 1989, Mol. MicroBiol. 3:1301-1306.
Payne & Finkelstein, 1977, "Imferon agar: Improved medium for isolation of pathogenic Nesseria." J. Clin. Microbiol. 6:293-297.
Pope & Payne, 1993, 93rd Am. Soc. Microbiol. Abstract B-147.
Pope et al., 1995, Infec. and Immun. 63(9):3642-3648.
Rappuoli et al., "Development of a vaccine against Helicobacter pylori: a short overview," European Journal of Gastroenterology & Hepatology, 5:576-578, 1993.
Robbins et al., 1991, "O-Specific side-chain toxin-protein conjugates as parentaeral vaccines for the prevention of shigellosis and related diseases." Rev. Inf. Dis. 13:S362-365.
H.W. Yoder, 1989, "Congo red binding by Escherichia coli isolates from chickens." Avian Dis. 33:502-505.
Sigma Chemical Co. Catalog, p. 420.
Sigma Chemical Co. Catalog, p. 105.
Frey Steven Michael
Pace John Lee
Walker Richard Ives
Antex Biologics Inc.
Naff David M.
Ware Deborah K.
LandOfFree
Methods for detecting Campylobacter bacteria or antibodies to Ca does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for detecting Campylobacter bacteria or antibodies to Ca, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for detecting Campylobacter bacteria or antibodies to Ca will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1850868