Methods for detecting bacteriophage MS2

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

Reexamination Certificate

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C435S005000, C435S004000, C435S007100

Reexamination Certificate

active

06841346

ABSTRACT:
The present invention relates to methods and assays for detecting bacteriophage MS2 in a sample.

REFERENCES:
Olive, D. M., and Bean, P. Principles and applications of methods for DNA-based typing of microbial organisms. J. Clin. Microbiol. 1999; 37: 1661-1669.*
Holland, P. M., Abramson, R. D., Watson, R. and Gelfand, D. H. Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity ofThermus aquaticus.Proc. Natl. Acad. Sci. 1991; 88:7276-7280.*
Livak KJ, Flood SJ, Marmaro J, Gius. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl. Jun. 1995;4(6):357-62.*
O'Connell et al. Application of Real-Time Fluorogenic Polymerase chain reaction (PCR) to nucleic acid-based detection of simulants and biothreat agents. 23rdArmy Science Conference, Dec. 2002.*
Printout of Genome disclosure by NCBI, Complete genome of Enterio bacteriophage MS2, Accession: NC 001417.*
Schweiger et al. Application of a Fluorogenic PCR Assay for Typing and Subtyping of Influenza Viruses in Respiratory Samples. Journal of Clinical Microbiology, Apr. 2000, p. 1552-1558, vol. 38, No. 4.

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