Methods and compositions for isolating nucleic acid sequence...

Details Methods and compositions for isolating nucleic acid sequence... Methods and compositions for isolating nucleic acid sequence...

2008-06-27

2009-12-22

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S022100, C536S023100, C536S024300

Reexamination Certificate

active

07635566

ABSTRACT:
The invention is drawn to isolating sequence variants of a genetic locus of interest using a modified iterative primer extension method. The nucleic acids analyzed are generally single stranded and have a reference sequence which is used as a basis for performing iterative single nucleotide extension reactions from a hybridized polymerization primer. The iterative polymerization reactions are configured such that polymerization of the strand will continue if the sequence of the nucleic acid being analyzed matches the reference sequence, whereas polymerization will be terminated if the nucleic acid being analyzed does not match the reference sequence. Nucleic acid strands that have mutations can be isolated using a variety of methods and sequenced to determine the precise identity of the mutation/polymorphism. By performing the method on both strands of the nucleic acid being analyzed, virtually all possible mutations can be identified.

REFERENCES:
patent: 5604097 (1997-02-01), Brenner
patent: 5635400 (1997-06-01), Brenner
patent: 5654413 (1997-08-01), Brenner
patent: 5763175 (1998-06-01), Brenner
patent: 5780231 (1998-07-01), Brenner
patent: 5846719 (1998-12-01), Brenner et al.
patent: 5863722 (1999-01-01), Brenner
patent: 5888737 (1999-03-01), DuBridge et al.
patent: 5962228 (1999-10-01), Brenner
patent: 6013445 (2000-01-01), Albrecht et al.
patent: 6103463 (2000-08-01), Chetverin et al.
patent: 6138077 (2000-10-01), Brenner
patent: 6140489 (2000-10-01), Brenner
patent: 6150516 (2000-11-01), Brenner et al.
patent: 6172218 (2001-01-01), Brenner
patent: 6175002 (2001-01-01), DuBridge et al.
patent: 6228589 (2001-05-01), Brenner
patent: 6235475 (2001-05-01), Brenner et al.
patent: 6265163 (2001-07-01), Albrecht et al.
patent: 6322971 (2001-11-01), Chetverin et al.
patent: 6352828 (2002-03-01), Brenner
patent: 6406848 (2002-06-01), Bridgham et al.
patent: 6511802 (2003-01-01), Albrecht et al.
patent: 6518023 (2003-02-01), Brenner
patent: 6654505 (2003-11-01), Bridgham et al.
patent: 6806052 (2004-10-01), Bridgham et al.
patent: 6831994 (2004-12-01), Bridgham et al.
patent: 6893822 (2005-05-01), Schweitzer et al.
patent: 6897023 (2005-05-01), Fu et al.
patent: 6969488 (2005-11-01), Bridgham et al.
patent: 7090804 (2006-08-01), Kayyem et al.
patent: 7282370 (2007-10-01), Bridgham et al.
patent: 7390632 (2008-06-01), Maihle et al.
patent: 2002/0051992 (2002-05-01), Bridgham et al.
patent: 2002/0061529 (2002-05-01), Bridgham et al.
patent: 2002/0137052 (2002-09-01), Bridgham et al.
patent: 2003/0027157 (2003-02-01), Fu et al.
patent: 2003/0032020 (2003-02-01), Brenner
patent: 2003/0049616 (2003-03-01), Brenner et al.
patent: 2003/0077615 (2003-04-01), Bridgham et al.
patent: 2003/0175702 (2003-09-01), Schweitzer et al.
patent: 2004/0002104 (2004-01-01), Fischer et al.
patent: 2004/0091857 (2004-05-01), Nallur et al.
patent: 2005/0059065 (2005-03-01), Brenner
patent: 2005/0176035 (2005-08-01), Crothers
patent: 2005/0181408 (2005-08-01), Brenner
patent: 2005/0186590 (2005-08-01), Crothers et al.
patent: 2005/0208576 (2005-09-01), Schweitzer et al.
patent: 2006/0051876 (2006-03-01), Bridgham et al.
patent: 2007/0219367 (2007-09-01), Shchepinov et al.
patent: 2008/0318796 (2008-12-01), Drmanac et al.
patent: 0969103 (2000-01-01), None
patent: 1693469 (2006-08-01), None
patent: WO96/12039 (1996-04-01), None
patent: WO/96/41011 (1996-12-01), None
patent: 99009211 (1999-02-01), None
patent: WO99/35293 (1999-07-01), None
patent: WO02/103011 (2002-12-01), None
patent: WO03/008638 (2003-01-01), None
patent: WO2005/080604 (2005-09-01), None
patent: 2005111244 (2005-11-01), None
patent: WO2006/086209 (2006-08-01), None
Till et al. High-throughput discovery of rare human nucleotide polymorphisms by Ecotilling. Nucleic Acids Research 34(13) : e99/1-e99/12 (2006).
Gerry et al., Universal DNA Microarray Method for Multiplex Detection of Low Abundance Point Mutations Journal of Molecular Biology 292(2) : 251-262 (1999).
Brenner et al. , In vitro cloning of complex mixtures of DNA on microbeads: physical separation of differentially expressed cDNAs. PNAS 97(4) : 1665-1670(2000).

Affiliated with

Also associated with

Rating

Methods and compositions for isolating nucleic acid sequence... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods and compositions for isolating nucleic acid sequence..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions for isolating nucleic acid sequence... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4112105