Methods and compositions for controlling plant pests

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers pathogen or pest resistance

Reexamination Certificate

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C435S069100, C435S252300, C435S410000, C435S320100, C536S023710, C536S023100, C800S300000

Reexamination Certificate

active

08076533

ABSTRACT:
Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-24 or the nucleotide sequence set forth in SEQ ID NO:1-12 and 25-44, as well as variants and fragments thereof.

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Ito, T. et al., “Activiation Process of the Mosquitocidal Delta-Endotoxin CRY39a Produced byBacillus thuringiensisSubsp. Aizawai BUN1-14 and Binding Property to Anopheles Stephensi BBMV”, Journal of Invertebrate Pathology, Sand Diego, CA, US, LNKD—DOI:10.1016/J. JIP.2006.05.007, vol. 93, No. 1, Sep. 1, 2006, pp. 29-35, XP024948627; ISSN: 0022-2011.
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