Method to enhance an immune response of nucleic acid...

Details Method to enhance an immune response of nucleic acid... Method to enhance an immune response of nucleic acid...

2003-07-07

2009-10-20

Chen, Shin-Lin (Department: 1632)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

C435S320100, C435S455000, C530S350000, C536S023500

Reexamination Certificate

active

07604803

ABSTRACT:
A composition for the co-delivery to a cell of a nucleic acid and an assistor protein, comprising vesicles, nucleic acid and protein, wherein the nucleic acid operatively encodes an antigenic protein or portion thereof which shares at least one epitope with the assistor protein, the composition comprising said nucleic acid and said assistor protein associated with the same vesicles as one another. A preferred embodiment is a composition comprising liposomes formed from liposome forming materials and, associated with the liposomes, nucleic acid operatively encoding an antigenic protein and an assistor protein, wherein the assistor protein shares at least one epitope with the antigenic protein. The composition is for use as a vaccine and provides improved immune response compared to non-vesicular compositions, or mixtures of liposomes some of which are associated with nucleic acid and some of which are associated with assistor protein.

REFERENCES:
patent: 5459127 (1995-10-01), Felgner et al.
patent: 6030619 (2000-02-01), Granoff et al.
patent: 6166177 (2000-12-01), Probst et al.
patent: 7008791 (2006-03-01), Gregoriadis et al.
patent: 7285289 (2007-10-01), Nagy et al.
patent: WO 97-28818 (1997-08-01), None
patent: WO 98-10748 (1998-03-01), None
patent: WO 99-30733 (1999-06-01), None
patent: WO 01/41739 (2001-06-01), None
patent: WO 01/56548 (2001-08-01), None
Pancer et al. (2006) Ann R. Immunology doi: 10.1146/annurev.immunol.24.021605.090542.
McCluskie et al., 1999, Molecular Medicine, vol. 5, p. 287-300.
Potter et al., 2004, Indian J Med Res, vol. 119, pp. 217-237.
Titti et al., 2007, Expert Opin. Emerging Drugs, vol. 12, No. 1, p. 23-48.
Gregoriadias et al., “Vaccine Entrapment in Liposomes”,Methods: A companion to Methods in Enzymology, Academic Press Inc., vol. 19, No. 1, Sep. 1999, pp. 156-162.
L. Alvarez-Lajonchere et al., “Additives and Protein-DNA Combinations Modulated the Humoral Immune Response Elicited by a Hepatitis C Virus Core-encoding Plasmid in Mice”,Mem Inst Oswaldo Cruz, Rio de Janeiro, vol. 97, No. 1, Jan. 2002, pp. 95-99.
G. Gregoriadis et al., “High Yield Incorporation of Plasmid DNA within Liposomes: Effect on DNA Integrity and Transfection Efficiency”,Journal of Drug Targeting, vol. 3, 1996, pp. 469-475.
M. Gürsel et al., “Immunoadjuvant action of plasmid DNA in liposomes”,Vaccine, vol. 17, 1999, pp. 1376-1383.
C. Kirby et al., “Dehydration-Rehydration vesicles: A Simple Method for High Yield Drug Entrapment in Liposomes”,Biotechnology, Nov. 1984, pp. 465-472.
D.M. Klinman et al., CpG motifs as immune adjuvants,Vaccine, vol. 17, 1999, pp. 19-25.
A. Lanzavecchia, “Antigen-specific interaction between T and B cells”,Nature, vol. 314, Apr. 11, 1985, pp. 537-539.
J. Senior et al., “Dehydration-rehydration vesicle methodology facilitates a novel approach to antibody binding to liposomes”,Biochimica et Biophysica Acta, vol. 1003, 1989, pp. 58-62.
B. Zadi et al., “A Novel Method for high-Yield Entrapment of Solutes into Small Liposomes”,Journal of Liposome Research, vol. 10, No. 1, 2000; pp. 73-80.

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