Method of purifying RNA using kosmotropic salt

Details Method of purifying RNA using kosmotropic salt Method of purifying RNA using kosmotropic salt

2011-04-12

2011-04-12

Lewis, Patrick T (Department: 1623)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S025410, C536S025420

Reexamination Certificate

active

07923551

ABSTRACT:
The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.

REFERENCES:
patent: 5234809 (1993-08-01), Boom et al.
patent: 2006/0270031 (2006-11-01), Hwang et al.
patent: 2007/0043216 (2007-02-01), Bair, Jr. et al.
patent: 2007/0092403 (2007-04-01), Wirbisky et al.
patent: 2007/0238109 (2007-10-01), Min et al.
patent: 2007/0269819 (2007-11-01), Kim et al.

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