Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Nonplant protein is expressed from the polynucleotide
Reexamination Certificate
2007-05-29
2007-05-29
Mehta, Ashwin D. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Nonplant protein is expressed from the polynucleotide
C800S287000
Reexamination Certificate
active
10130150
ABSTRACT:
The invention provides a method of producing a recombinant polypeptide having β-glucosidase enzymatic activity, a polynucleotide encoding the polypeptide, nucleic acid constructs carrying the polynucleotide, transformed cells, such as yeast cells, and transgenic organisms expressing the polynucleotide. The invention provides various uses of the polypeptide, the polynucleotide, cells and/or organisms, including producing a recombinant polypeptide having β-glucosidase enzymatic activity, increasing the level of aromatic compounds in alcoholic beverages, as well as other fermentation products of plant material, hydrolyzing cellobiose and thus increasing the level of fermentable glucose, increasing the production of alcohol; such as ethanol from plant material, increasing the aroma released from a plant, or a plant, product, and hydrolysis or transglycosylation of glycosides.
REFERENCES:
patent: 3791932 (1974-02-01), Schuurs et al.
patent: 3839153 (1974-10-01), Schuurs et al.
patent: 3850578 (1974-11-01), McConnell et al.
patent: 3850752 (1974-11-01), Schuurs et al.
patent: 3853987 (1974-12-01), Dreyer
patent: 3867517 (1975-02-01), Ling
patent: 3879262 (1975-04-01), Schuurs et al.
patent: 3901654 (1975-08-01), Gross
patent: 3935074 (1976-01-01), Rubenstein et al.
patent: 3984533 (1976-10-01), Uzgiris
patent: 3996345 (1976-12-01), Ullman et al.
patent: 4034074 (1977-07-01), Miles
patent: 4098876 (1978-07-01), Piasio et al.
patent: 4879219 (1989-11-01), Wands et al.
patent: 5011771 (1991-04-01), Bellet et al.
patent: 5281521 (1994-01-01), Trojanowski et al.
patent: 5316931 (1994-05-01), Donson et al.
patent: 5863783 (1999-01-01), Van Heuvel et al.
patent: 5997913 (1999-12-01), Fowler et al.
patent: 6015703 (2000-01-01), White et al.
patent: 0307071 (1989-03-01), None
Goodall GJ and Filipowicz W. The minimum functional length of pre-mRNA introns in monocots and dicots. (1990) PMB vol. 14, pp. 727-733.
IwashitaApsergillus kawachiiDNA for beta-D-glucosidase, complete cds. (1999) GenBank Accession AB003470.
Dan s, Marton I, Dekel M. Bravdo B-A, He S, Withers SG, and Shoseyov O. Cloning, Expression, Characterization and Nucleophile Identification of Family 3,Aspergillus nigerbeta-Glucosidase. (2000) JBC vol. 275, pp. 4973-4980.
Vaquero C, Sack M, Chandler J, Drossard J, Schuster F, Monecke M, Schillberg S, and Rischer R. Transient expression of a tumore-specific single-chain fragment and a chimeric antibody in tobacco leaves. (1999) PNAS vol. 96, pp. 11128-11133.
Leah R, Kigel J, Svendsen I, and Mundy J. Biochemical and Molecular Characterization of a Barley Seed beta-Glucosidase. (1995) JBC vol. 270, pp. 15789-15797.
Bovy AG, Angenent GC, Dons HJM, and van Altworst A-C. Heterologous expression of the Arabidopsis etr1-1 allele inhibits the senescence of carnation flowers. (1999) Molecular Breeding vol. 5, pp. 301-308.
Le Traon-Masson et al, “Purification and Characterization of two β-D-glucosidases from anAspergillus nigerenzyme preparation: affinity and specificity toward glucosylated compounds characteristics of the processing of fruits”, (1998) Enz. Micro. Technol., 22 (5) 374-382.
Penttila et al, “Cloning ofAspergillus nigerGenes in Yeast. Expression of the Gene CodingAspergillusBeta-Glucosidase”, Mol. Gen. Genet. 194, 494-499, 1984.
Dekker R.F.H., “Kinetic, Inhibition, and Stability Properties of a Commercial Beta-D Glucosiade Cellobiase Preparation fromAspergillus-nigerand its Suitability in the Hydrolysis of Lignocellulose”, Biotechnology and Bioengineering, vol. 28, No. 9, 1986, pp. 1438-1442, XP002000249, ISSN: 0006-3592, *the whole document*.
Zhou s. et al; “Engineering Endoglucanase-secreting Strains of Ethanologenic Klebsiella Oxytoca P2.” Journal of Industrial Microbiology & Biotechnology, vol. 22, No. 6, Jun. 1999, pp. 600-607, XP000971439, ISSN: 1367-5435, *the whole document*.
Shoseyov O. et al. “Immobilized endo-beta-glucosidase enriches flavour of wine and passion fruit juice” Journal of Agricultural and Food Chemistry, vol. 38, No. 6, 1990, pp. 1387-1390, XP001122152, ISSN: 0021-8561, *the whole document*.
Iwashita Kazuhiro et al. “The bg1A gene ofAspergillus kawachiiencodes both extracellular and cell wall-bound beta-glocosidases”, applied and envrionmental Microbiology, vol. 65, No. 12, Dec. 1999, pp. 5546-5553, XP002228145, ISSN: 0099-2240, *figure 3*.
Siegel et al., -& Database EMBL (online), Feb. 18, 2000, retrieved from EBI, database accession No. AJ132386, XP002228148.
Bhat M.K. et al. “Cellulose Degrading Enzymes and Their Potential Industrial Applications.” Biotechnology Advances, vol. 15, No. 3-4, 1997, pp. 583-620, XP004089630, ISSN: 0734-9750, p. 604, paragraph 3.
Iwashita et al. database access. No. AB003470.
Kawaguchi et al. database access. No. P48825.
Iwashita et al. database access. No. BAA19913.
Shoseyov et al, “Endo-β-Glucosidase fromAspergillus NigerGrown on a Monoterpine Glycoside Containing Medium”, (1988), Phytochem. 27(7), 1973-1976.
Yeoh, et al, “Kinetic Properties of β-glucosidase fromAspergillus ornatus”, (1986), Appl. Microbiol. Biotechnol. 25, 25-28.
Zarevucka et al, “A Chemoenzymatic Synthesis of theO-Glycosides”, (1998) Chirality 10, 676-68.
Yi et al, “A novel approach to biotransformations in aqueous-organic two-phase systems: enzymatic synthesis of alkyl beta-[D]-glucosides using microencapsulated beta-glucosidase”, Biotechnol Bioeng. Nov. 5, 1998;60(3):385-90.
Henrissat et al, “Updating the sequence-based classification of glycosyl hydrolases”, Biochem J. Jun. 1, 1996;316 (Pt 2):695-6.
Hrmova et al, Barley beta-D-glucan exohydrolases with beta-D-glucosidase activity. Purification, characterization, and determination of primary structure from a cDNA clone, J Biol Chem. Mar. 1, 1996;271(9):5277-86.
Y. Gueguen, et al. “A Very Efficient Beta-Glucosidase Catalyst for the Hydrolysis of Flavor Precursors of Wines and Fruit Juices”, J. Agric. Food chem. 44:2336-2340, 1996.
Shoseyov et al, Immobilized Endo-β-glucosidase Rnriches Flavor of Wine and Passion Fruit Juice, (1990), J. Agric. Food. Chem. 39, 1387-1390.
Prade et al, “Enzymatic sysnthesis of disaccharides usingAgrobacteriumsp. B-glucosidase”, (1998) Carbohyd. Res. 305, 371-381.
Watanabe et al, “Purification and Properties ofAspergillus nigerβ-glucosidase”, (1992), Eur. J. Biochem. 209, 651-659.
Wong et al, “Identification of Glu-540 as the catalytic nucleophile of human beta-glucuronidase using electrospray mass spectrometry”, J Biol Chem. Dec. 18, 1998;273(51):34057-62.
Clark et al,, “Qualitative and quantative analysis of flavor additives on tobacco products using SPME-GC-Mass Spectroscopy”. J. Aagric. Food Chem. 45 (3), 844-849, 1997.
Shana et al, “Cloning and Characterization of Elongation Specific endo-1,4-β-glucanase (cell) fromArabidopsis thaliana”, Plant Mol. Biol., 34:837-842, 1997.
Parodi, AJ, “Role of N-oligosaccharide endoplasmic reticulum processing reactions in glycoprotein folding and degradation”, Biochem J., May 15, 2000;348 Pt 1:1-13.
Ben-Ami Bravdo
Dekel Mara
Marton Ira
Shoseyov Oded
Shu Wei
Kingdon Worley Cathy
Mehta Ashwin D.
Yissum Research Development Company of the Hebrew University of
LandOfFree
Method of producing recombinant Aspergillus niger... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of producing recombinant Aspergillus niger..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of producing recombinant Aspergillus niger... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3733108