Method of producing polyunsaturated fatty acids, novel...

Details Method of producing polyunsaturated fatty acids, novel... Method of producing polyunsaturated fatty acids, novel...

2002-01-18

2009-11-10

McElwain, Elizabeth F (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

C435S320100, C435S252300, C536S023200

Reexamination Certificate

active

07615679

ABSTRACT:
The present invention relates to a method for the production of unsaturated fatty acids with at least two double bonds. The invention further relates to the use of the nucleic acid sequence of SEQ ID NO: 1 encoding a polypeptide having delta-5 desaturase activity in the method and for generating a transgenic organism, preferably a transgenic plant or microorganism with an increased content of fatty acids, oil or lipids with unsaturated long chain fatty acids, to their homologs or derivatives, and to gene constructs encompassing these genes. The invention also relates to the use of the nucleic acid sequences alone or in combination with further desaturases and/or elongase genes in the method and for generating transgenic organisms.

REFERENCES:
patent: 5614393 (1997-03-01), Thomas et al.
patent: 6043411 (2000-03-01), Nishizawa et al.
patent: 2004/0049805 (2004-03-01), Lerchl et al.
patent: 0550162 (1993-07-01), None
patent: 0794250 (1997-09-01), None
patent: WO-91/13972 (1991-09-01), None
patent: WO-93/06712 (1993-04-01), None
patent: WO-93/11245 (1993-06-01), None
patent: WO-94/11516 (1994-05-01), None
patent: WO-94/18337 (1994-08-01), None
patent: WO-95/18222 (1995-06-01), None
patent: WO-96/13591 (1996-05-01), None
patent: WO-96/21022 (1996-07-01), None
patent: WO-97/21340 (1997-06-01), None
patent: WO-97/30582 (1997-08-01), None
patent: WO-98/46765 (1998-10-01), None
patent: WO-99/27111 (1999-06-01), None
Broun et al, Science 282: 1315-1317, Nov. 13, 1998.
Van de Loo et al, PNAS, USA 92: 6743-6747, Jul. 1995.
Doerks et al, TIG 14(6): 248-250, Jun. 1998.
Smith et al, Nature Biotechnology 15:1222-1223, Nov. 15, 1997.
Brenner, S.E., TIG 15(4): 132-133, Apr. 1999.
Bork et al, TIG 12(10): 425-427, Oct. 1996.
DeLUca, V., AgBiotech News and Information 5(6): 225N-229N, 1993.
Stukey, Joseph E., et al., “The OLE1 Gene ofSaccharomyces cerevisiaeEncodes the Δ9 Fatty Acid Desaturase and Can Be Functionally Replaced by the Rat Stearoyl-CoA Desaturase Gene”, The Journal of Biological Chemistry, vol. 265, No. 33, 1990, pp. 20144-20149.
Wade, Hajime, et al., “Enhancement of chilling tolerance of a cyanobacterium by genetic manipulation of fatty acid desaturation,” Nature, vol. 347, 1990, pp. 200-203.
Huang, Yung-Sheng, et al., “Cloning of Δ12- and Δ6-Desaturases fromMortierella alpinaand Recombinant Production of γ-Linolenic Acid inSaccharomyces cerevisiae”, Lipids, vol. 34, No. 7, 1999, pp. 649-659.
McKeon et al., “Stearoyl-Acyl Carrier Protein Desaturase from Safflower Seeds”, Methods in Enzymology, vol. 71, Lipids, Part C, Academic Press, NY, 1981, pp. 275-281.
Wang, X.-M., et al., “Synthesis and regulation of linolenic acid in higher plants”, Physiol. Biochem., vol. 26, No. 6, 1988, pp. 777-792.
Napier, J.A., et al., “Identification of aCaenorhabditis elegansΔ6-fatty-acid-desaturase by heterologous expression inSaccharomyces cerevisiae”, Biochem J., vol. 330, 1998, pp. 611-614.
Obukowicz, Mark G., et al., “Identification and Characterization of a Novel Δ6/Δ5 Fatty Acid Desaturase Inhibitor As a Potential Anti-Inflammatory Agent”, Biochemical Pharmacology, 1998, vol. 55, pp. 1045-1058.
Moreno, Victor J., at al., “Biosynthesis of Unsaturated Fatty Acids in the DiatomPhaeodactylum tricomutum”, Lipids, vol. 14, 1979, No. 1, pp. 15-19.
Abbadi, Amine, et al., “Transgenic oilseeds as sustainable source of nutritionally relevant C20 and C22 polyunsaturated fatty acids?”, Eur. J. Lipid Sci. Technol., vol. 103, 2001, pp. 106-113.
Domergue, Frédéric, at al., “Cloning and functional characterization ofPhaeodactylum tricomutumfront-end desaturases involved in eicosapentaenoic acid biosynthesis”, Eur. J. Biochem., vol. 269, 2002, pp. 4105-4113.

Affiliated with

Also associated with

Rating

Method of producing polyunsaturated fatty acids, novel... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of producing polyunsaturated fatty acids, novel..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of producing polyunsaturated fatty acids, novel... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4098257