Method of producing a biological extracellular matrix for use as

Prosthesis (i.e. – artificial body members) – parts thereof – or ai – Implantable prosthesis

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623 16, 128DIG8, A61F 202

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059162651

ABSTRACT:
Tissue is procured from a human or animal donor. The tissue may be fixed by application of a fixative. Cellular components which would cause rejection are removed from tissue by a chemical treatment that allows the extracellular matrices (ECM) to retain their original shapes, biological structures and ultrastructures, locations and durability. The resulting ECM serves as a cell scaffold on which living cells are seeded to form a lifegraft for implanting or for allograft reconstruction.

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patent: 5112354 (1992-05-01), Sires
Osamu Ohtani Three dimensional Organization of the Connective Tissue Fibers of the Human Pancreas: A Scanning electron Microscopic Study of NaOH treated tissues vol. 5 pp. 557-566.
O'Donnel et al: An alkali Digestion method to expose connective Tissue Fibers: A Scanning electron Microscopic Study of Rat lung (Journal of Electron Microscopy technique 19: 486-496 (1991).
Schneider et al: An improved method for endothelid cell seeding on polytetrafluoroethylene small caliber vascular grafts.

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