Method of measuring a co-enzyme

Details Method of measuring a co-enzyme Method of measuring a co-enzyme

1990-09-19

1992-01-14

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving urea or urease

435 14, 435 16, 435 18, 435 26, 435 28, C12Q 158, C12Q 154, C12Q 152, C12Q 134

Patent

active

050810146

ABSTRACT:
The method makes use of the action of the co-enzyme in the production of H.sub.2 O.sub.2 associated with the reaction of a hydroxylase with a decoupling agent in the presence of air or oxygen, the H.sub.2 O.sub.2 being subsequently determined by quantitative breaking of the C--F bond of a fluorinated compound in the presence of peroxidase, followed by electrometric titration of the resulting ions.

REFERENCES:
patent: 4271265 (1981-06-01), Deneke et al.
patent: 4353983 (1982-10-01), Siddiqi
The Merck Index, Tenth Edition, 1983, p. 762.
White-Stevens et al., J. Biol. Chem., vol. 247, 1972, pp. 2358.

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