Method of making cysteine depleted IL-6 muteins

Details Method of making cysteine depleted IL-6 muteins Method of making cysteine depleted IL-6 muteins

1994-03-10

1996-08-13

Draper, Garnette D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 43525233, 4353201, 935 22, 935 52, 935 72, 536 235, C12N 1524, C07K 1454

Patent

active

055455379

ABSTRACT:
Muteins of IL-6 and truncated IL-6 are prepared by recombinant DNA techniques. In the muteins, the cysteine residues that occur at positions, or at positions corresponding to positions, 45 and 51 of mature, native IL-6 have been replaced by other amino acids. The cysteine residues that occur at positions, or at positions corresponding to positions, 74 and 84 are retained. The molecule has biological activity that is at least comparable to that of native IL-6.

REFERENCES:
patent: 4853332 (1989-08-01), Mark et al.
Snouwaert et al., Journal of Immunology 146, 585-591 (1991).
Brakenhoff et al., Journal of Immunology 145, 561-568 (1990).
Clogston et al., Archives of Biochemistry and Biophysics 272, 144-151 (1989).
Brakenhoff et al., Journal of Immunology 143, 1175-1182 (1989).
Brakenhoff et al., Journal of Immunology 139, 4116-4121 (1987).
Asagoe et al., Biotechnology 6, 806-809 (1988).
Yasueda et al., Biotechnology 8, 1036-1040 (1990).
"Altered Sites in vitro Mutagenesis System Technical Manual," Promega Corporation.
Sugasarawa et al., Biotechnology 6, 895-902 (1988).

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