Method of designing primer and probe sets, primer and probe...

Details Method of designing primer and probe sets, primer and probe... Method of designing primer and probe sets, primer and probe...

2006-03-06

2010-02-02

Kim, Young J (Department: 1637)

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Biological or biochemical

C435S006120, C435S091100, C435S091200

Reexamination Certificate

active

07657382

ABSTRACT:
A method of designing primer and probe sets for identification of target sequences by amplification and hybridization is provided. Also, primer and probe sets designed by the method, a kit comprising the sets, a computer readable medium recorded thereon a program to execute the method, and a method of identifying target sequences using the sets are provided. Accordingly, primer and probe sets capable of identifying rapidly and accurately a number of target sequences can be readily designed.

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Notredame, C., et al., “T-Coffee: A Novel Method for Fast and Accurate Multiple Sequence Alignment,” J. Mol. Biol. (2000) vol. 302,pp. 205-217.
Chenna, R., et al., “Multiple Sequence Alignment With The Clustal Series Of Programs,” Nucleic Acids Research, (2003) vol. 31, No. 13, pp. 3497-3500.
Edgar, Robert C., “MUSCLE: Multiple Sequence Alignment With High Accuracy And High Throughput,” Nucleic Acids Research, (2004) vol. 32, No. 5, pp. 1792-1797.

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