Method of culturing animal cells

Details Method of culturing animal cells Method of culturing animal cells

1990-09-27

1993-10-05

Elliott, George C.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524026, 4352402, 435813, 43524027, C12N 502, C12N 506, C12N 512, C12N 516

Patent

active

052504328

ABSTRACT:
A method of culturing animal cells, which comprises (A) subjecting living animal cells to suspension culture in a culture tank, (B) withdrawing a portion of a suspension culture fluid containing the living animal cells from the culture tank, (C) supplying the withdrawn suspension culture fluid to a centrifugal separating device which is operated under specific conditions and separating the living animal cells from the suspension culture fluid, (D) withdrawing the separated living animal cells from the centrifugal separating device, and (E) recycling at least a portion of the withdrawn living animal cells to the culture tank for the step (A).

REFERENCES:
patent: 4673650 (1987-06-01), Braden
patent: 4900669 (1990-02-01), Hatch et al.
Le Rumeur et al, "Albumin Section and Protein Synthesis by Cultured Diploid and Tetraploid Rat Hepatocytes Separated by Elutriation" Exp. Cell Res. V. 147 247-254, 1983.
Arkhiopv, "Cultivation of Cells on liquid fluorocarbon substrates" Chem. Abstracts, vol. 96, #100292 p. 359, 1982.
Patent Abstracts of Japan, 13(42) (C-564) [3390] (Jan. 30, 1989).

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