Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including freezing; composition therefor
Patent
1999-08-02
2000-10-24
Saucier, Sandra E.
Chemistry: molecular biology and microbiology
Differentiated tissue or organ other than blood, per se, or...
Including freezing; composition therefor
435 11, A01N 102
Patent
active
061365254
ABSTRACT:
An artificial liver support system is described herein which comprises cryopreserved hepatocytes having an initial viability of 80-99%. Further disclosed are hepatocytes cryopreserved by dispensing hepatocytes into freezing containers, freezing the containers from between minus 50 to minus 90 degrees Celsius, storing the containers in liquid or vapor nitrogen, thawing the cryopreserved hepatocytes when ready for use and removing residual cryoprotectant media.
REFERENCES:
Chesne et al., "Viability and Function in Primary Culture of Adult Hepatocytes from Various Animal Species and Human Beings after Cryopreservation", Hepatology 18 (2):406-414 (1993).
Cain Shawn Paul
Jauregui Hugo O.
Mullon Claudy Jean-Paul
Naik Sharda
Perlman Timothy Jon
Circe Biomedical, Inc.
Saucier Sandra E.
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