Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2005-09-06
2009-10-27
Ramirez, Delia M (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C435S189000, C435S193000, C435S069100, C435S252320, C435S440000, C435S006120, C435S026000, C435S015000, C530S350000, C536S023200
Reexamination Certificate
active
07608437
ABSTRACT:
A method of producing coryneform bacteria having an improved amino acid or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium so that it is close to a consensus sequence or introducing a change in the promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on the chromosome of a coryneform bacterium by gene recombination so that it is close to a consensus sequence, obtaining mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows one of skill in the art to construct a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield, by the recombination or mutation.
REFERENCES:
patent: 6255086 (2001-07-01), Kuwabara et al.
patent: 6881566 (2005-04-01), Kuwabara et al.
patent: 2002/0045223 (2002-04-01), Suga et al.
patent: 2004/0002143 (2004-01-01), Asakura et al.
patent: 0 771 879 (1997-05-01), None
patent: 61-104790 (1986-05-01), None
patent: 63-214189 (1988-09-01), None
patent: 6-502548 (1994-03-01), None
patent: 2708168 (1997-10-01), None
patent: WO 93/03158 (1993-02-01), None
patent: WO 00/18935 (2000-04-01), None
Ko et al., GenBank accession No. AF030520, 1997.
Branden et al., Introduction to Protein Structure, Garland Publishing Inc., New York, p. 247, 1991.
Witkowski et al., Biochemistry 38:11643-11650, 1999.
Voet and Voet, Biochemistry, John Wiley & Sons, New York, 1990, pp. 523, 525.
Eikmanns et al., Microbiology 140:1817-1828, 1994.
Bormann et al., Applied and Environmental Microbiology 59(7):2329-2331, 1993.
Official Communication from Korean Intellectual Property Office dated Nov. 23, 2005 and its English translation.
Bormann et al., GenBank accession No. X59404, 1992.
Eikmanns et al., Genbank accession No. X66112, 1994.
Nakamura et al, GenBank accession No. AB025424, Apr. 1999.
Eikmanns et al., GenBank accession No. X71489, 1995.
Lewin, Genes IV, pp. 225-226, 1990.
Bork, Genome Research, 10: 398-400, 2000.
Broun et al, Science, 282: 1315-1317,1998.
Seffemick et al., J. Bacteriol., 183(3): 2405-2410, 2001.
Van de Loo et al, Proc. Natl. Acad. Sci., 92:6743-6747, 1995.
Baggio et al, GenEMBL accession No. BTU82241, Jan. 11, 1997.
Teller et al, GenEMBL accession No. CLOSGDHG, Jun. 30, 1993.
B. Fournier, et al, “Strength and Regulation of The Different Promoters for Chromosomal β-Lactamases of Klebsiella Oxytoca”, Antimicrobial Agents and Chemotherapy, vol. 43, No. 4, Apr. 1999, p. 850-855.
T. Jeffke. et al., “Mutational Analysis of the CBB Operon (CO2Assimilation) Promoter of Ralstonia Eutropha”, Journal of Bacteriology, vol. 181, No. 14, Jul. 1999, p. 4374-4380.
C. Guerrero, et al, Gene, vol. 138, pp. 35-41, “Directed Mutagenesis of a Regulatory Palindromic Sequence Upstream From the Brevibacterium Lactofermentum Tryptophan Operon”, 1994.
K. Sano, et al., Gene, vol. 53, pp. 191-200, “Structure and Function of the TRP Operon Control Regions of Brevibacterium Lactofermentum, A Glutamic-Acid-Producing Bacterium”, 1987.
E.M.T. El-Mansi, “Control of Metabolic Interconversion of Isocitrate Dehydrogenase Between The Catalytically Active and Inactive Forms inEscherichia Coli”, FEMS Microbiology Letters, 166, (1998), 333-339.
E. Alvarez-Villafane, et al., “Two NAD”-Isocitrate Dehydrogenase Forms in Phycomyces Blakesleeanus. Induction in Response to Acetate Growth and Characterization, Kinetics, and Regulation of Both Enzyme Forms, Biochemistry, 1996, 36, 4741-4752.
R.F. Cadenas, et al., Gene, vol. 98, pp. 117-121. “Construction and Characterization of Promoter-Probe Vectors for Corynebacteria Using the Kanamycin-Resistance Reporter Gene.” 1991.
A. Cid, et al. Correct Genetics, vol. 12, pp. 105-110. “Replacement of the Promoter of the Yeast Plasma Membrane ATPase Gene by a Galactose-Dependent Promoter and Its Physiological Consequences,” 1987.
R.E. Dalbey, et al., The Journal of Biological Chemistry, vol. 260, No. 29, pp. 15925-15931, “Leader Peptidase Catalyzes the Release of Exported Proteins From the Outer Surface of theEscherichia ColiPlasma, Membrane,” Dec. 15, 1965.
G. Miozzari, et al., Proc. Natl. Acad. Sci, vol. 75, No. 11. pp. 5580-5584, “Naturally Occurring Promoter Down Mutation: Nucleotide Sequence of theTRPPromoter/Operator/Leader Region of Shigella Dysenteriae 16,” Nov. 1978.
Y. Morinaga, et al., Journal of Biotechnology, vol. 5, pp. 305-312, “Expression ofEscherichia ColiPromoters in Brevibacterium Lactofermentum Using the Shuttle Vector pEB003,” 1987.
M. Patek, et al., Microbiology, vol. 142, pp. 1297-1309, “Promoters From Corynebacterium Glutamicum: Cloning, Molecular Analysis and Search for a Consensus Motif,” 1996.
O. Raibaud, et al., Gene, vol. 29, pp. 231-241, “A Technique for Integrating any DNA Fragment into the Chromosome ofEscherichia Coli” 1984.
M. Rosenberg, et al., Ann. Rev. Genet. vol. 13, pp. 319-353, “Regulatory Sequences Involved in the Promotion and Termination of RNA Transcription,” 1979.
H. Shimotsu, et al., Gene, vol. 43, pp. 85-94, Construction of A Single-Copy Integration Vector and its use in Analysis of Regulation of theTRPOperon of Bacillus Subtilis.
S. Sugimoto, et al., Journal of Biology, vol. 5, pp. 237-253, “Hyperproduction of Phenylalanine byEscherichia Coli: Application of a Temperature-Controllable Expression Vector Carrying the Repressor-Promoter System of Bacteriophage Lambda,” 1987.
P. Youderian, et al., Cell, vol. 30, pp. 843-853, “Sequence Determinants of Promoter Activity,” Oct. 1982.
T.J. Zupancic, et al., FEMS Microbiology Letters, vol. 131, pp. 121-126, “Isolation of Promoters from Brevibacterium Flavum Strain MJ233C and Comparison of their Gene Expression Levels in B. Flavum andEscherichia Coli,” 1995.
Bernhard J. Eikmanns et al., “Nucleotide sequence, expression and transcriptional analysis of theCorynebacterium glutamicum gltAgene encoding citrate synthase”, Microbiology, 140, pp. 1817-1828, 1994.
Elke R. Bormann et al., “Glutamate Dehydrogenase Is Not Essential for Glutamate Formation byCorynebacterium glutamicum”, Applied and Environmental Microbiology, 59, 7, pp. 2329-2331, Jul. 1993.
U.S. Appl. No. 09/648,482, Suga et al., filed Aug. 28, 2000.
Asakura Yoko
Ito Hisao
Kanno Sohei
Kimura Eiichiro
Kurahashi Osamu
Ajinomoto Co. Inc.
Cermak Shelly Guest
Cermak Kenealy Vaidya & Nakajima LLP
Ramirez Delia M
LandOfFree
Method of constructing amino acid producing bacterial... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of constructing amino acid producing bacterial..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of constructing amino acid producing bacterial... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4078000