Method of amplifying a target nucleic acid by rolling circle...

Details Method of amplifying a target nucleic acid by rolling circle... Method of amplifying a target nucleic acid by rolling circle...

2011-08-23

2011-08-23

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C435S091520

Reexamination Certificate

active

08003322

ABSTRACT:
Provided is kit for and a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase,(d) an endonuclease, (e) a DNA polymerase, and (f) a primer.

REFERENCES:
patent: 6498023 (2002-12-01), Abarzua
patent: 2001/0039039 (2001-11-01), Weissman et al.
patent: 2005/0069939 (2005-03-01), Wang et al.
patent: 2007/0015182 (2007-01-01), Abarzua
patent: 2007/0031857 (2007-02-01), Makarov et al.
patent: 2007/018601 (2007-02-01), None
Alsamadi et al. “High Accuracy Genotyping Directly from Genomic DNA using a Rolling Circle Amplification Based Assay.” BMC Genomics, Biomed Central, Great Britian vol. 4, No. 1, May 2003, pp. 1-18.

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