Method for the recovery of extracellular enzymes from whole ferm

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435183, 435202, 435814, 435816, C12N 956, C12N 900, C12N 928

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active

047286130

ABSTRACT:
Extracellular enzymes can be recovered from a whole fermentation beer by adding to the whole beer a mixture of a polymer and an inorganic salt. This total mixture produces an enzyme-rich polymer phase and a cell debris-containing, enzyme-poor salt phase which can be separated to produce an enzyme-rich product from the polymer phase.

REFERENCES:
patent: 3415804 (1968-12-01), Poison
patent: 3819528 (1974-06-01), Berry
patent: 4144130 (1979-03-01), Kula et al.
patent: 4508825 (1985-04-01), Kim et al.
patent: 4591563 (1986-05-01), Paul et al.
"Protein Recovery Using Two-Phase Systems", Hustedt et al., Trends in Biotechnology, vol. 3, No. 6, pp. 139-144, 1985.

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