Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2008-07-01
2008-07-01
Nashed, Nashaat T. (Department: 1656)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S414000, C435S069100, C435S194000, C435S320100
Reexamination Certificate
active
11000867
ABSTRACT:
A method for the recombinant production and purification of a protein kinase selected from the group consisting of tyrosine protein kinases and serine/threonine kinases by expressing a nucleic acid encoding said kinase in a microbial host cell, forming inclusion bodies containing said kinase, isolating, solubilizing, naturing, and purifying said kinase wherein said purification is performed by hydrophobic interaction with an hydrophobic adsorbent under conditions whereby at least 70% of said protein kinase are not bound to said adsorbent and the protein kinase not bound to said adsorbent is recovered.
REFERENCES:
patent: 5336615 (1994-08-01), Bell et al.
patent: 6005081 (1999-12-01), Burton et al.
patent: 6184360 (2001-02-01), Burton et al.
patent: 6423831 (2002-07-01), Burton et al.
patent: 6812330 (2004-11-01), Burton et al.
patent: 6900034 (2005-05-01), Suck et al.
patent: 2005/0249701 (2005-11-01), Morre et al.
patent: WO 03/016516 (2003-02-01), None
patent: WO 03/097669 (2003-11-01), None
Werber, M.M., et al., 1991, “Large-scale purification and refolding of recombinant proteins derived from fibronectin domains”, Biology of Recombinant Microorganism and Animal Cells, vol. 34 (Meeting), pp. 369-382.
Arakawa, T. et al., 1993, “Production and characterization of an analog of acidic fibroblast growth factor with enhanced stability and biological activity”, Protein Engineering, vol. 6, No. 5, pp. 541-546.
Breton, J., et al., 1995, “Structure, stability, and biological properties of a N-terminally truncated form of recombinant human interleukin-6 containing a single disulfide bond”, European Journal of Biochemistry, vol. 227, pp. 573-581.
Oda, Y., et al., 1999, “cSrc is a major cytosolic tyrosyne kinase in vascular tissue”, Canadian Journal of Physiology and Pharmacology, vol. 77, No. 8, pp. 606-617.
Moellering, B.J., et al., 1999, “Folding and purification of a recombinantly expressed interferon regulatory factor, IRF-4”, Protein Expression and Purification, vol. 16, pp. 160-170.
Thies, M.J.W., & Pirkl, F., 2000, “Chromatographic purification of the CH2 domain of the monoclonal antibody MAK33”, Journal of Chromatography B, vol. 737, pp. 63-69.
Johnson, T.M., et al., 2000, “Expression of functional recombinant scorpion beta-neurotoxin Css II inE. coli”, Peptides, vol. 21, pp. 767-772.
Hsieh, M.-C., & Cooperman, B.S., 2000, “The preparation and catalytic properties of recombinant human prostate-specific antigen (rPSA)”, Biochimica et Biophysica Acta, vol. 1481, pp. 75-87.
Guo, L.-A., 2001, “Simultaneous purification and renaturation of recombinant human interferon-alpha expressed byE. coliby high-performance hydrophobic interaction chromatography”, Chinese Journal of Chromatography, vol. 19, No. 4, pp. 301-303 and English language abstract.
Ferre, H., et al., Mar. 2003, “Purification of correctly oxidized MHC class I heavy-chain molecules under denaturaing conditions: A novel stratgy exploiting disulfice assisted protein folding”, vol. 12, pp. 551-559.
2002 Amersham Biosciences product catalogue, pp. 544, 545, and 549.
Brazil, D.P. & Hemmings, B.A.,Trends Biochem. Sci., 11 (2001) 657-664.
Clark, E.D.,Curr. Opin. Biotechnol., 12 (2001) 202-207.
Datta, S.R. et al,Genes Dev.13 (1999) 2905-2927.
Egan, S.E. & Weinberg, R.A.,Nature365 (1993) 781-783.
Hardie, G., & Hanks, S.,The Protein Kinase Facts Books I, Academic Press, 1995, pp. 7-20.
Hunter, T.,Cell50 (1987) 823-829.
Lee, S.Y,TrendsBiotechnol.14 (1996) 98-105.
Lilie, H.,Current Opinion Biotechnol., 9 (1998) 497-501.
Mattes, R.,Semin. Thromb. Hemost., 27 (2001) 325-335.
Misawa, S. & Kumagai, I.,Biopolymers51 (1999) 297-307.
Panda, A.K. et al,Biotechnol., 75 (1999) 161-172.
Thomas, S.M. & Brugge, J.S.,Annu. Rev. Cell Dev. Biol.13 (1997) 513-609.
Clark, E.D.,Current Opin. In Biotech., 9:2 (1988) 157-162.
Ouellette T. et al,Protein Expression and Purification, Academic Press, 30:2, (Aug. 2003) 156-166.
Caspers, P. et al,Cellular and Molecular Biology, CMB Assoc., 40:5 (1994) 635-644.
Keesler, G.A., et al,Protein Expression and Purification, Academic Press, 14:2 (1998) 221-228.
Jullien, P. et al,European Journal of Biochemistry, 224:2 (1994) 589-596.
Shi et al,Biotechniques, 23:6 (1997) 1036-1038.
Suttnar, J. et al,Journal of chromatography B: Biomedical Appl., Elsevier Sci. Publishers, NL, 656:1 (1994) 123-126.
Lin, W.J. et al,Protein Expression and Purification, 4:3 (1993) 256-264.
Pav, S. et al,Protein Sci, 6:1 (1997) 242-245.
Li Ming, et al.,Separation and identification of different refolding components, Journal of Biotechnology, vol. 103, No. 2, pp. 119-127 (2003), XP002321231.
Hertenberger Hubert
Honold Konrad
Klein Christian
Rueger Petra
Hoff Hoffmann-La Roche Inc.
Johnston George W.
Moore William W.
Nashed Nashaat T.
Rocha-Tramaloni Patricia S.
LandOfFree
Method for the recombinant production and purification of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the recombinant production and purification of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the recombinant production and purification of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3936209