Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-11-08
2011-12-06
Woolwine, Samuel (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200
Reexamination Certificate
active
08071310
ABSTRACT:
A method for the identification of a gene in a transposon population is provided. The method comprises isolating genomic DNA, optionally pooling the DNA, restricting the DNA in the pools using an enzyme, ligating adaptors, amplifying the adaptor-ligated fragments with primers one of which is a primer complementary to a border of a transposon sequence, sequencing the fragments using high throughput sequencing, aligning the fragments with known sequences in a database and thereby identifying gene candidates.
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Michiel Vandenbussche, et al., “Toward the Analysis of thePetuniaMADS Box Gene Family by Reverse and Forward Transposon Insertion Mutagenesis Approaches: B, C, and D Floral Organ Identity Functions Require Sepallata-Like MADS Box Genes inPetunia”, The Plant Cell, vol. 15, No. 11, Nov. 2003, pp. 2680-2693.
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Gerats Antonius Gerardus Marie
van Eijk Michael Josephus Theresia
van Tunen Adrianus Johannes
Vandenbussche Michiel Marcel Albert
Browdy and Neimark PLLC
Keygene NV
Woolwine Samuel
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