Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1993-07-23
2000-11-28
Minnifield, Nita
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4242561, 435 697, 4352528, 43525233, 4351723, C12N 1509, C12N 120, C12P 2104, A61K 39102
Patent
active
061534064
ABSTRACT:
The present invention relates, in general, to a method of expressing the outer membrane protein P2 from Haemophilus influenzae type b (Hib-P2) and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein P2 from Haemophilus influenzae type b or fusion protein thereof in E. coli wherein the Hib-P2 protein or fusion protein comprises more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of Hib-P2 protein and fusion protein thereof.
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Nu
Blake Milan S.
Liang Shu-Mei
Pullen Jeffrey K.
Soper Thomas
Tai Joseph Y.
Minnifield Nita
North American Vaccine, Inc.
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