Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-05-24
2009-08-18
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200
Reexamination Certificate
active
07575864
ABSTRACT:
Methods for the direct detection of diagnostic target RNA have been developed, which obviate the need for time consuming RNA purification and isolation procedures.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 2002/0026046 (2002-02-01), Pasloske et al.
patent: 2003/0170617 (2003-09-01), Pasloske
patent: 2004/0197833 (2004-10-01), Loessner
patent: WO 91/02817 (1991-03-01), None
patent: WO 03/008636 (2003-01-01), None
patent: WO 03/008636 (2003-01-01), None
Chiocchia et al. Highly sensitive method to detect mRNAs in individual cells by direct RT-PCR using Tth DNA polymerase. BioTechniques (1997) 22:312-318.
Sonja Selenska -Pobell, Detection of mRNA and rNA Via Reverse Transcription and PCR in Soil. Molecular Microbial Ecology Manual 2.7.5, 1995, pp. 1-14.
Sanjay Tyagi et. al., Molecular Beacons: Probes That Fluoresce Upon Hybridization. Nature Biotechnology, 1996, pp. 303-308, vol. 14.
Carola Burtscher et. al., Evaluation of the Use of PCR and Reverse Transcriptase PCR for Detection of Pathogenic Bacteria in Biosolids From Anaerobic Digestors and Aerobic Composters, Applied and Environmental Microbiology, 2003, pp. 4618-4627, vol. 69.
Wilson, Development of Sensitive, High-Throughput One-Tube RT-PCR-Enzyme Hybridisation Assay to Detect Selected Bacterial Fish Pathogens, Diseases of Aquatic Organisms, 2003, pp. 127-134, vol. 54.
M.J. Loessner et. al., A New Procedure for Efficient Recovery of DNA, RNA, and Proteins From Listeria Cells by Rapid Lysis With a Recombinant Bacteriophage Endolysin, Applied Environmental Microbiology, 1995, pp. 1150-1152, vol. 61.
Ririe et. al., Product Differentiation by Analysis of DNA Melting Curves During the Polymerase Chain Reaction, Anal. Biochem., 1997, pp. 154-160, vol. 245.
J. Sambrook et. al., Molecular Cloning, 1989, A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (Book Not Included).
T.J. Silhavy et. al., Experiments With Gene Fusions, 1984, Cold Spring Laboratory, Cold Springs, NY (Book Not Included).
F.M. Ausubel et. al., Current Protocols in Molecular Biology, 1987, Greene Publishing Assoc. and Wiley-Interscience, NY. (Book Not Included).
P. Gerhardt et. al., Manual of Methods for General Bacteriology, American Society for Microbiology, Washington, DC., 1994 (Book Not Included).
T.D. Brock, Biotechnology: A Textbook of Industrial Microbiology, 2nd Edition, Sinauer Associates, Inc, Sunderland, MA, 1989 (Book Not Included).
Collins, M. D., U. Rodrigues, et al. (1991). “Phylogenetic analysis of the genusLactobacillusand related lactic acid bacteria as determined by reverse transcriptase sequencing of 16S rRNA.” FEMS Microbiology Letters 77(1):5-12.
de Chateau, M. and L. Bjorck (1996). “Identification of interdomain sequences promoting the Intronless evolution of a bacterial protein family.” Proc Natl Acad Sci U S A 93(16):8490-5.
Valtilingom, M., F. Gendre, et al. (1998). “Direct detection of viable bacteria, molds, and yeasts by reverse transcriptase PCR in contaminated milk samples after heat treatment.” Appl Environ Microbiol 64(3): 1157-60.
el, Y., J. M. Lee, et al. (2001). “High-density microarray-mediated gene expression profiling ofEscherichla coli.” J Bacteriol 183(2): 545-56.
Vaitilingom M et al: “Direct Detection of Viable Bacteria, Molds, and Yeast by Reverse Transcriptase PCR in Contaminated Milk Samples After Heat Treatment” Applied and Environmental Microbiology, Washington, D.C., US, vol. 64, No. 3, Mar. 1998, pp. 1157-1160.
Wei Yan et al: “High-Density MMicroarray-Mediated Gene Expression Profiling ofEscherichia coli” Journal of Bacteriology, Washington, D.C., vol. 183, No. 2, Jan. 2001, pp. 545-556.
Loessner MJ et al: “A New Procedure for Efficient Reovery of DNA, RNA, and Proteins From Listeria Cell by Rapid Lysis With a Recombinant Bacteriophage Endolysin” Applied and Environmental Microbiology, Washington, D.C., vol. 61, No. 3, Mar. 1995, pp. 1150-1152.
Chateau De M et al: “Identification of Interdomain Sequences Promoting the Intronless Evolution of a Bacterial Protein Family” Proceedings of the National Academy of Sciences of USA, National Academy of Science, Washington, D.C., vol. 93, Aug. 1996, pp. 8490-8495.
Collins M D et a;: “Phylogenetic Analysis of the GenusLactobacillusand Related Lactic Acid Bacteria as Determined by Reverse Transcriptase Sequencing of 16S RRNA” FEMS Microbiology Letters, Amsterdam, NL., vol. 77, No. 1, 1991, pp. 5-12.
Collins, M. D., U. Rodrigues, et al. (1991). “Phylogenetic analysis of the genusLactobacillusand related lactic acid bacteria as determined by reverse transcriptase sequencing of 16S rRNA.” FEMS Microbiology Letters 77(1): 5-12.
de Chateau, M. and L. Bjorck (1996). “Identification of interdomain sequences promoting the intronless evolution of a bacterial protein family.” Proc Natl Acad Sci U S A 93(16): 8490-5.
Loessner, M. J., A. Schneider, et al. (1995). “A new procedure for efficient recovery of DNA, RNA, and proteins from Listeria cells by rapid lysis with a recombinant bacteriophage endolysin.” Appl Environ Microbiol 61(3): 1150-2.
Vaitilingom, M., F. Gendre, et al. (1998). “Direct detection of viable bacteria, molds, and yeasts by reverse transcriptase PCR in contaminated milk samples after heat treatment.” Appl Environ Microbiol 64(3): 1157-60.
Wei, Y., J. M. Lee, et al. (2001). “High-density microarray-mediated gene expression profiling ofEscherichia coli.” J Bacteriol 183(2): 545-56.
International Search Report and Written Opnion of the International Searching Authority PCT/US2005/018838, Mailed Dec. 1, 2006.
Walz G, Zanker B, (1989) Sequential Effects of interleukin 2 -diptheria toxin fusion protein on T cell Activation, Proc. Natl. Acad. Sci. USA 86(23):9485-88.
QuantumRNA beta Actin Internal Standards Kit (Cat. #1720), Instruction Manual Version 0607 ,(2006) Ambion/Applied Biosystems, Austin, TX USA.
Linear Acyrlamide Cat # AM9520, Product Insert (2006) Ambion/Applied Biosystems, Austin, TX USA.
Application Note, MELT Total Nucleic Acid Isolation System: a new technology for hands free tissue disruption, RNA preservation and total nucleic acid purification, Nature Methods 2: 2005.
Weigers U., and Hilz H., (1971) A new method of using “Proteinase K” to prevent mRNA degradation during isolation from HeLa Cells, Biochem. Biophys. Res. Com 44(2):513-519.
Bedzyk Laura
DeMarco Daniel
Ebersole Richard
Jackson Raymond
Varkey Stephen
E.I. du Pont de Nemours and Company
Horlick Kenneth R.
Thomas David C
LandOfFree
Method for the direct detection of diagnostic RNA does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for the direct detection of diagnostic RNA, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the direct detection of diagnostic RNA will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4084259