Chemistry: analytical and immunological testing – Including sample preparation – Liberation or purification of sample or separation of...
Patent
1992-07-17
1996-09-03
Czaja, Donald E.
Chemistry: analytical and immunological testing
Including sample preparation
Liberation or purification of sample or separation of...
436178, 422 70, 422 72, 422101, 435272, 210657, 210660, G01N 118
Patent
active
055523250
ABSTRACT:
Disclosed is a centrifuge tube containing a porous selection means for selectively separating and recovering biological substances from gels, broths, or solutions containing the same, and thus concentrating them, the tube comprising separate upper and lower containers and means for joining the two containers to form a unitary device, the bottom of the upper container comprising a porous selection means, preferably a membrane containing a particulate substance which will selectively bind the biological substances while allowing unbound substances to pass through from the upper to lower container while being centrifuged. A method for recovering the biological substances in high concentration using the centrifuge tube is also provided, comprising introducing said gels, broths, or solutions containing the desired biological substances, centrifuging the same until the desired substance is bound to the selection means, and eluting the bound material from the selection means to recover the substance in concentrated form.
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Goldberg Bruce S.
Nochumson Samuel
Czaja Donald E.
Elden Richard E.
FMC Corporation
Greenfield Mark A.
Ramstad Polly E.
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