Method for separating rennet components

Details Method for separating rennet components Method for separating rennet components

1987-08-17

1988-05-17

Jones, Raymond N.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435183, 426 36, 426 42, 426 63, 426271, 530416, C12N 964, A23C 912, A23C 1902

Patent

active

047450630

ABSTRACT:
Substantially pure chymosin is separated from rennet extracts by contacting a liquid extract with an anionic exchange medium, preferably a diethylaminoethyl (DEAE) cellulose resin packed in a liquid chromatographic column, either as a static bed or stirred bed. The rennet extract and resin are conditioned so that the resin selectively binds pepsin and chymosin passes through the resin bed and is recovered from the liquid extract from the column. The bound pepsin is periodically removed from the resin by passing a suitable eluant through the resin bed.

REFERENCES:
patent: 344433 (1886-06-01), Blumenthal
patent: 3281332 (1966-10-01), Munns
patent: 3573277 (1971-03-01), Grant
patent: 3766015 (1973-10-01), Dardas
patent: 4136201 (1979-01-01), Feldman
patent: 4526868 (1985-07-01), Shasuzzaman et al.

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