Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-11-12
1998-11-03
Myers, Carla J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 4, 435 912, 435 72, 435 15, 436 34, 436 63, 436 64, 436 94, 436501, 935 77, 935 78, C12Q 168, C12P 1934
Patent
active
058306447
ABSTRACT:
Method and compositions are provided for the determination of telomere length and telomerase activity, as well as the ability to increase or decrease telomerase activity in the treatment of proliferative diseases. Particularly, primers are elongated under conditions which minimize interference from other genomic sequences, so as to obtain accurate determinations of telomeric length or telomerase activity. In addition, compositions are provided for intracellular inhibition of telomerase activity and means are shown for slowing or reversing the loss of telomeric repeats in aging cells.
REFERENCES:
patent: 4737454 (1988-04-01), Dattagupta et al.
patent: 5489508 (1996-02-01), West et al.
Rhyu, M., Journal National Cancer Institute (Jun. 1995) 87:884-894.
Grieder and Blackburn, "The Telomere Terminal Transferase of Tetrahymena Is a Ribonucleoprotein Enzyme with Two kinds of Primer Specificity", 51 Cell 887, 1987.
Blackburn et al., "Recognition and elongation of telomeres by telomerase", 31 Genome 553, 1989.
Greider, "Telomerase Is Processive", 11 Molec. and Cell. Biology 4572, 1991.
Counter et al., "Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity", 11 EMBO 1921, 1992.
Harley, "Telomere loss: mitotic clock or genetic time bomb?", 256 Mutation Res. 271, 1991.
Eck and Nabel, "Antisense oligonucleotides for therapeutic intervention", 2, Opin. Biotech 897, 1991.
Allsopp et al., "Telomere length predicts replicative capacity of human fibroblasts," Proc. Natl. Acad. Sci. USA 89:10114-10118 (1992).
Harley, "Telomere Loss: Mitotic Clock or Genetic Time Bomb?" 256 Nature 271, 1991.
Zahler, et al. "Inhibition of Telomerase by G-quartet DNA Structures", 350 Nature 718, 1991.
Blackburn, et al., "Recognition and Elongation of Telomeres by Telomerase", 31 Genome 553, 1989.
Yu et al., "Developmentally Programmed Healing of Chromosomes by Telomerase in Tetrahymena", 67 Cell 823, 1991.
Harley et al., "Telomeres Shorten During Ageing of Human Fibroblasts" 345 Nature 458, 1990.
Blackburn, "Structure and Function of Telomeres", 350 Nature 572, 1991.
Cech, "Ribozymes and Their Medical Implications", 260 Jnl of Amer. Med. Assoc. 3030, 1988.
Greider and Blackburn, "A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis," Nature 337:331-337 (1989).
Cotten, "The in vivo application of ribozymes," Trends in Biotechnology 8:174-178 (1990).
Harley et al., "The Telomere Hypothesis of Cellular Aging," Expermiental Gerontology 27:375-382 (1992).
Shay et al., "Loss of telomeric DNA during aging may predipose cells to cancer (Review)," Int'l J. Oncology 3:559-563 (1993).
Windle and McGuire, "Telomeres: the long and the short of it," Proceedings of the American Association for Cancer Research 33:594-595 (1992).
Counter et al., "Stabilization of Short Telomeres and Telomerase Activity Accompany Immortalization of Epstein-Barr Virus-Transformed Human B Lymphocytes," J. Virology 68:3410-3414 (1994).
Klingelhutz et al., "Restoration of Telomeres in Human Papoillomavirus-Immortalized Human Anogenital Epithelial Cells," Molecular and Cellular Biology 14:961-969 (1994).
Counter et al., "Telomerase activity in human ovarian carcinoma," Proc. Natl. Acad. Sci. USA 91:2900-2904 (1994).
Strahl and Blackburn, "The effects of nucleoside analogs on telomerase and telomeres in Tetrahymena," Nucleic Acids Research 22:893-900 (1994).
S. Wang and V. Zakian (1990) Nature 345:456. Telomere-telomere recombination provides an express pathway for telomere acquisition.
S. Goldstein (1990) Science 249:1129. Replicative senescence: the human fibroblast comes of age.
J. Smith and R. Whitney (1980) Science 207:82. Intraclonal variation in proliferative potential of human diploid fibroblasts:stochastic mechnisms for cellular aging.
T. Ohno (1979) Mechanisms of aging and development 11:179. Strict relationship between dialyzed serum concentration and cellular life span in vitro.
L. Hayflick and P. Moorhead (1961) Experimental Cell Research 25:585. The serial cultivation of human diploiid cell strains.
J. Szostak (1989) Nature 337:303. The beginning of the ends.
G. Jankovic, et al. (1991) Nture 350:197. Telomere loss and cancer.
J. Gall (1990) Nature 344:108. Tying up loose ends.
G. Yu, et al. (1990) Nature 344:126. In vivo alteration of telomere sequences and renescence caused by mutated Tetrahymena telomerase RNAs.
L. Harrington and C. Greider (1991) Nature 353:451. Telomerase primer specificity and chromosome healing.
J. Gray, et al. (1991) Cell 67:807. Cloning and expression of genes for the Oxytricha telomere-binding protein:specific subunit interactions in the telomeric complex.
F. Muller, et al. (1991) Cell 67:815. New telomere formation after developmentally regulated chromosomal breakage during the process of chromosome diminution in Ascaris lumbricoides.
G. Yu and E. Blackburn (1991) Cell 67:823. Developmentally programmed healing of chromosomes by telomerase in Tetrahymena.
E. Blackburn, et al. (1989) Genome 31:553. Recognition and elongation of telomeres by telomerase.
C. Greider (1990) Bioessays 12:363. Telomeres, telomerase and senesence.
E. Henderson, et al. (1990) Biochemistry 29:732. Telomere G-strand structure and function analyzed by chemical protection, base analogue substitution, and utilization by telomerase in vitro.
D. Gottschling, et al. (1990) Cell 63:751. Position effect at S. cerevisiae telomeres: reversible repression of Pol II transcription.
V. Lundblad and J. Szostak (1989) Cell 57:633. A mutant with a defect in telomere elongation leads to senescence in yeast.
E. Blackburn (1984) Annual Reviews in Biochemistry 53:163. The molecular structure of centromeres and telomeres.
A. Olovnikov (1973) J. Theoretical Biology 41:181. A theory of marginotomy.
E. Blackburn (1991) Nature 350:569. Structure and function of telomeres.
H. Cooke and B. Smith (1986) CSHSQB LI:213. Variability at the telomeres of the human X/Y pseudoautosomal region.
C. Greider (1991) Cell 67:645. Chromosome first aid.
G. Morin (1989) Cell 59:521. The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats.
C. Harley, et al. (1990) Nature 345:458. Telomeres shorten during ageing of human fibroblasts.
Ham and McKeehan (1979) Methods in Enzymology LVIII:44. Media and growth requirements.
J. Starling, et al. (1990) Nucleic Acids Research 18:6881. Extensive telomere repeat arrays in mouse are hypervariable.
Shay Jerry
West Michael D.
Wright Woodring E.
Board of Regents , The University of Texas System
Geron Corporation
Hellenkamp Amy S.
Kaster Kevin
Myers Carla J.
LandOfFree
Method for screening for agents which increase telomerase activi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for screening for agents which increase telomerase activi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for screening for agents which increase telomerase activi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-687851