Method for removing fumarase activity, microorganisms obtainable

Details Method for removing fumarase activity, microorganisms obtainable Method for removing fumarase activity, microorganisms obtainable

1998-10-28

2000-08-15

Prouty, Rebecca E.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Enzyme inactivation by chemical treatment

435106, 435116, 435822, 435830, 435848, 435874, 435800, 435820, 435109, 435128, C12P 1300, C12N 904

Patent

active

061035084

ABSTRACT:
This invention relates to a method for removing fumarase activity from a microorganism or processed product thereof having ethylenediamine-N,N'-disuccinic acid ethylenediamine lyase activity, which includes treating the microorganism or processed product thereof with an aqueous alkaline solution at a pH of 8.0 to 10.5 in the presence of at least one salt with a concentration of 5 mM to 1000 mM. The salt is preferably selected from the group consisting of sodium, potassium, ammonium and C.sub.2-6 alkanediamine salts of boric acid, phosphoric acid, hydrochloric acid, sulfuric acid, acetic acid, oxalic acid, fumaric acid, maleic acid and ethylenediamine-N,N'-disuccinic acid, and mixtures thereof. This invention also relates to a microorganism or processed product thereof having reduced fumarase activity obtainable by the above described method, and to a method for producing an optically active aminopolycarboxylic acid from fumaric acid and a compound having amino group in the presence of the microorganim or processed product thereof having reduced fumarase activity as a catalyst.

REFERENCES:
patent: 5116743 (1992-05-01), Goto et al.
Guest et al., Journal of Bacteriology, Feb. 1983, pp. 588-596.
Witschel et al., Biodegradation, vol. 8, No. 6, pp. 419-428, (1998) .

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