Method for purifying dermostatin A and dermostatin B

Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...

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C07D31300

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051590026

ABSTRACT:
The present invention provides a method for treating patients suffering from the acquired immunedeficiency syndrome (AIDS). The method comprises administering dermostatin, dermostatin A, dermostatin B or derivatives thereof, such as pharmaceutically acceptable esters, salts or complexes. The administration of the dermostatin or dermostatin A or dermostatin B should be done as soon as possible after the detection of the presence of the HIV virus which is believed to be responsible for the onset of AIDS. These polyene macrolide antibiotics have been found to be effective in improving the viability of lumphocyte cells infected with the HIV virus and in preventing the replication of the virus. The present invention also provides for the first time substantially pure dermostatin A and dermostatin B and an HPLC method for separation of dermostatin complex into those components in substantial purity.

REFERENCES:
patent: 4743695 (1988-05-01), Schmitz et al.
patent: 4863955 (1989-09-01), Moore et al.
R. Pandey et al., The Journal of Antibiotics, "Polyene Antibiotics," vol. XXVI, No. 8 pp. 475-477 (1973).
R. Pandey, Hindustan Antibiotics Bulletin, "The Structures of Dermostatin A and B," 22 (3 & 4), pp. 47-61 (1980).
H. Willard et al., "Instrumental Methods of Analysis" 6th ed., pp. 529-530 and 536-543, D. Van Nostrand Co. New York (1981).
N. Narasimhachari et al., Chemical Abstracts 65:5301c, "Purification of dermostatin . . . " (1965).
M. Windholz et al., "The Merck Index" 10th ed. entry 2280, p. 420, Merck & Co., Rahway, N.J. (1983).
R. Pandey et al., Chemical Abstracts 79:146360v, "Polyene Antibiotics," p. 311 (1973).

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