Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1993-06-11
1998-11-10
Guzo, David
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
4351723, 435369, 435372, 435373, 4353201, C12P 1934, C12N 510, C12N 1563, C12N 1586
Patent
active
058342567
ABSTRACT:
The invention provides a novel retroviral packaging system, in which retroviral packaging constructs and packagable vector transcripts are produced from high expression plasmids by transfection in human cells. High titers of recombinant retrovirus are produced in infected cells. The methods of the invention include the use of the novel retroviral constructs to transduce primary human cells, including T cells and bone marrow stem cells, with foreign genes by cocultivation at high efficiencies. The invention is useful for the rapid production of high viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.
REFERENCES:
patent: 4405712 (1983-09-01), Vande Woude et al.
patent: 4650764 (1987-03-01), Temin et al.
patent: 4861719 (1989-08-01), Miller
patent: 5024939 (1991-06-01), Gorman
A. Dusty Miller, Nature, vol. 357, "Human gene therapy comes of age," 1 Jun. 1992, pp. 455-460.
Watanabe et al., Molecular and Cellular Biology, vol. 3, No. 12, "Construction of a Helper Cell Line for Avian Reticuloendotheliosis Virus Cloning Vectors," Dec. 1983, pp. 2241-2249.
Mann et al., Cell, vol. 33, "Construction of a Retrovirus Packaging Mutant and Its Use to Produce Helper-Free Defective Retrovirus," 1983, pp. 153-159.
Embretson et al., Virology, vol. 61, No. 9, "Lack of Competition Results in Efficient Packaging of Heterologous Murine Retroviral RNAs and Reticuloendotheliosis Virus Encapsidation-Minus RNAs by the Reticuloendotheliosis Virus Helper Cell Line," Sep. 1987, pp. 2675-2683.
Cloyd et al., The Journal of Experimental Medicine, vol. 151, "Lumphomagenicity of Recombinant Mink Cell Focus-Inducing Murine Leukemia Viruses," 1980, pp. 542-552.
Donahue et al., The Journal of Experimental Medicine, vol. 176, "Helper Virus Induced T Cell Lymphoma in Nonhuman Primates after Retroviral Mediated Gene Transfer," Oct. 1992, pp. 1125-1135.
Cone et al., Proc. Natl. Acad. Sci. USA, vol. 81, "High-efficiency gene transfer into mammalian cells: Generation of helper-free recombinant retrovirus with broad mammalian host range," Oct. 1984, pp. 6349-6353.
Miller et al., Molecular and Cellular Biology, vol. 6, No. 8, "Redesign of Retrovirus Packaging Cell Lines To Avoid Recombination Leading to Helper Virus Production," Aug. 1986, pp. 2895-2902.
Miller et al., BioTechniques, vol. 7, No. 9, "Improved Retroviral Vectors for Gene Transfer and Expression," 1989, pp. 980-990.
Bosselman et al., Molecular and Cellular Biology, vol. 7, No. 5, "Replication-Defective Chimeric Helper Proviruses and Factors Affecting Generation of Competent Virus: Expression of Moloney Murine Leukemia Virus Structural Genes via the Methallothionein Promoter," May 1987, pp. 1797-1806.
Markowitz et al., Journal of Virology, vol. 62, No. 4, "A Safe Packaging Line for Gene Transfer: Separating Viral Genes on Two Different Plasmids," Apr. 1988, pp. 1120-1124.
Danos et al., Proc. Natl. Acad. Sci. USa, vol. 85, "Safe and effiient generation of recombinant retroviruses with amphotropic and ecotropic host ranges," Sep. 1988, pp. 6460-6464.
Belmont et al., Molecular and Celular Biology, vol. 8, No. 12, "Expression of Human Adenosine Deaminase in Murine Hematopoietic Cells," Dec. 1988, pp. 5116-5125.
Dhawan et al., Science, vol. 254, "Systemic Delivery of Human Growth Hormone by Injection of Genetically Engineered Myoblasts," 6 Dec. 1991, pp. 1509-1511.
Yao et al., Proc. Natl. Acad. Sci. USA, vol. 88, "Expression of human factor IX in rat capillary endothelial cells: Toward somatic gene therapy for hemophilia B," Sep. 1991, pp. 8101-8105.
Armentano et al., Proc. Natl. Acad. Sci. USA, vol. 87, "Expression of human factor IX in rabbit hepatocytes by retrovirus-mediated gene transfer: Potential for gene therapy of hemophilia B," Aug. 1990, pp. 6141-6145.
Morecki et al., Cancer Immunol Immunother, vol. 32, "Retrovirus-mediated gene transfer into CD4+ and CD8+ human T cell subsets derived from tumor-infiltrating lymphocytes and peripheral blood mononuclear cells," 1991, pp. 342-352.
van Beusechem et al., Proc. Natl. Acad. Sci. USA, vol. 89, "Long-term expression of human adenosine deaminase in rhesus monkeys transplanted with retrovirus-infected bone-marrow cells," Aug. 1992, pp. 7640-7644.
Apperley et al., Blood, vol. 78, No. 2, "Retroviral Gene Transfer of Human Adenosine Deaminase in Murine Hematopoietic Cells: Effect of Selectable Marker Sequences on Long-Term Expression," Jul. 15, 1991, pp. 310-317.
Landau et al., Journal of Virology, vol. 66, No. 8, "Packaging System for Rapid Production of Murine Leukemia Virus Vectors with Variable Tropism," Aug. 1992, pp. 5110-5113.
Heinzel et al., Journal of Virology, vol. 62, No. 10, "Use of Simian Virus 40 Replication To Amplify Epstein-Barr Virus Shuttle Vectors in Human Cells," Oct. 1988, pp. 3738-3746.
Chesebro et al., "Failure of Human Immunodeficiency Virus Entry and Infection in CD4-Positive Human Brain and Skin Cells", Journal of Virology, 64(1):215-221, (1990).
Donahue, et al., "Helper Virus Induced T Cell Lymphoma in Nonhuman Primates after Retroviral mediated Gene Transfer", J. of Experimental Medicine, 176:1125-1135, (1992).
Landau et al., "Pseudotyping with Human T-Cell Leukemia Virus Type I Broadens the Human Immunodeficiency Virus Host Range", Journal of Virology, 65(1):162-169, (1991).
Miller et al., "Construction and Properties of Retrovirus Packaging Cells Based on Gibbon Ape Leukemia Virus", Journal of Virology, (65)5:2220-2224, (1991).
Pedersen et al., "Feline Leukemia Virus Infection as a Potentiating Cofactor for the Primary and Secondary Stages of Experimentally Induced Feline Immunodeficiency Virus Infection", Journal of Virology, 64(2):598-606, (1990).
RNA Tumor Viruses, Cold Spring Harbor Laboratory, pp. 26-30, 262-270, 371-380, 393-423, (1984).
van Beusechem et al., "Long-Term Expression of Human Adenosine Deaminase in Rhesus Monkeys Transplanted with Retrovirus-Infected Bone-Marrow Cells", Proc. Natl. Acad. Sci., USA, 89:7640-7644, (1989).
Johnson et al., "A Lethal Myeloproliferative Syndrome in Mice Transplanted with Bone Marrow Cells Infected with a Retrovirus Expressing Granulocyte-Macrophage Colony Stimulating Factor", THE EMBO Journal, 8(2):441-448, (1989).
Cepko et al., "Construction and Applications of a Highly Transmissible Murine Retrovirus Shuttle Vector", Cell, 37:1053-1062, (1984).
Amentano et al., "Effect of Internal Viral Sequences on the Utility of Retroviral Vectors", Journal of Virology, 61(5):1647-1650, (1987).
Miller et al., "Generation of Helper-Free Amphotropic Retroviruses That Transduce a Dominant-Acting, Methotrexate-Resistant Dihydrofolate Reductase Gene", Molecular and Cellular Biology, 5(3):431-437, (1985).
Boshart et al., "A Very Strong Enhancer Is Located Upstream of an Immediate Early Gene of Human Cytomegalovirus", Cell, 41;521-530, (1985).
Miller et al., "Improved Retroviral Vectors for Gene Transfer and Expression", BioTechniques, 7(9):980-989, (1989).
Finer et al. "kat: A High-Efficiency Retroviral Transduction System for Primary Human T Lymphocytes" Blood, vol. 83, No. 1 (Jan. 1) 1994, pp. 43-50.
Dull Thomas J.
Farson Deborah A.
Finer Mitchell H.
Qin Lu
Roberts Margo R.
Cell Genesys Inc.
Guzo David
LandOfFree
Method for production of high titer virus and high efficiency re does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for production of high titer virus and high efficiency re, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for production of high titer virus and high efficiency re will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1515561