Method for producing somoclonal variant cotton plants

Details Method for producing somoclonal variant cotton plants Method for producing somoclonal variant cotton plants

1995-06-07

1999-02-23

Fox, David T.

Multicellular living organisms and unmodified parts thereof and

Method of chemically, radiologically, or spontaneously...

435418, 435427, 4354301, 435431, 800265, 800268, 800270, 800298, 800301, 800314, C12N 1501, A01H 104, A01H 106, A01H 400

Patent

active

058746625

ABSTRACT:
A method for producing somaclonal variant cotton plant. The method comprising providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant, culturing the undifferentiated callus in callus growth medium supplemented with sucrose as primary carbon source until embryogenic callus is formed from the undifferentiated callus, transferring the embryogenic callus to a plant germination medium, culturing the embryogenic callus on the plant germination medium until a plantlet is formed from the embryogenic callus, transferring the plantlets to soil, growing the plantlets to produce seeds from self pollination, collecting the seeds, planting the seeds, growing the seeds under conditions to select for a desired characteristic and collecting the plants with the desired characteristics.

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