Method for producing recombinant trypsin

Details Method for producing recombinant trypsin Method for producing recombinant trypsin

2007-10-02

2007-10-02

Bragdon, Kathleen Kerr (Department: 1656)

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

C435S069100, C435S226000, C435S254110, C435S320100, C536S023200

Reexamination Certificate

active

10470508

ABSTRACT:
The present invention concerns a method for producing recombinant trypsin from porcine pancreas inPichia pastoriswhich is soluble and secreted into the culture medium, whereby expression at pH 3.0-4.0 substantially prevents activation of trypsinogen to β-trypsin and autolysis of β-trypsin by α-trypsin into ε-trypsin and from there into inactive peptides.

REFERENCES:
patent: 5945328 (1999-08-01), Woldike et al.
patent: 6159722 (2000-12-01), Bode et al.
patent: 6420157 (2002-07-01), Darrow et al.
patent: 6458564 (2002-10-01), Darrow et al.
patent: 6485957 (2002-11-01), Darrow et al.
patent: 7029909 (2006-04-01), Uemura et al.
patent: 2003/0157634 (2003-08-01), Mattanovich et al.
patent: 2003/0207402 (2003-11-01), Kopetzki et al.
patent: 249282 (1987-09-01), None
patent: 0 597 681 (1993-10-01), None
patent: 0 699 687 (1995-08-01), None
patent: 1012307 (2000-06-01), None
patent: 11-507207 (1999-06-01), None
patent: WO97/00316 (1997-01-01), None
patent: WO99/10503 (1999-03-01), None
patent: WO00/17332 (2000-03-01), None
patent: WO 01/19970 (2001-03-01), None
patent: WO01/55429 (2001-08-01), None
Vestling, M.M., Murphy, C.M., Fenselau, C. “Recognition of Trypsin Autolysis Products by High-Performance Liquid Chromatography and Mass Spectrometry”, Anal. Chem. 1990, 62, 2391-2394.
Walker, J.E., Keil, B., “Purification and Characterisation of Different Active Forms of Pork Trypsin”, Ear. J. Biochem, 32, 486-491, (1973).
Rice, R.H., Means, G.E., Brown, W.D., “Stabilization of Bovine Trypsin by Reductive Methylation”, Biochimica et Biophysica Acta, 492 (1977) 316-321.
Hedstrom, L., Szilagyi, L., Rutter, W.J., “Converting Trypsin to Chymotrypsin: The Role of Surface Loops”, Science, vol. 255, Mar. 6, 1992, pp. 1249-1253.
Brodrick, J.W., Largman C., Johnson, J.H., Geokas, M.C., “Human Cationic Trypsinogen-Purification, Characterization, and Characteristics of Autoactivation”, The Journal of Biological Chemistry, vol. 263, No. 8, Apr. 26, 1978, pp. 2732-2736.
Keil, B., “Trypsin”, The Enzymes vol. II, 3rdEdition, Acad. Press N.Y., 1971, pp. 249-275.
XP-00223000, publication date: Jul. 21, 1986, p. 1-3.
XP-002171427, publication date: Jul. 21, 1986, p. 1-4.
Boyer, Paul D., “The Enzymes,” USA, Academic Press, 1971, 249-275.
Cregg et al., Biotechnology, 1993, 11(8), 905-910.
Hedstrom, L. et al., “Converting Tyrpsin to Chymotrypsin: The Role of Surface Loops,” Reports, Mar. 6, 1992, 1249-1253.
Emi, Mitsuru et al., Cloning, Characterization and Nucleotide Sequences of Two cDNAs Encoding Human Pancreatic Trypsinogens, Gene 41 (1986), 305-310.
Vasquez, J. et al., “An Expression System for Trypsin,” Cell. Biochemistry 39:265-276 (1989).
Selecting aPichiaExpression Vector, Invitrogen technical document, vector map pPICZalpha A, B, C.
Sharp, P. et al., “The Codon Adaptation Index—A Measure of Directional Synonymous Codon Usage Bias, And Its Potential Applications,” Nucleic Acids Research vol. 15, No. 3, 1987, 1281-1295.
Liener, I.E., “Chromatographic Studies on Trypsin, Trypsinogen And The Activation Process,” Biochem. Biophys. 88 (1960), 216-221.
Mettler-Toledo, pH Control In Fermentation Plants, Application Notes, Aug. 2004.
Graf, L. et al., “Selective Alteration of Substrate Specificity by Replacement of Aspartic Acid-189 with Lysine in the Binding Pocket of Trypsin,” Biochemistry, vol. 26, (1987), 2616-2623.

Affiliated with

Also associated with

Rating

Method for producing recombinant trypsin does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for producing recombinant trypsin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for producing recombinant trypsin will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3830264