Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1987-10-26
1990-09-18
Griffin, Ronald W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435207, 435254, 435911, 435913, 435918, 536 11, 536123, 536124, C12P 1904, C12N 938, C12N 114, C07H 300
Patent
active
049578600
ABSTRACT:
The present invention provides a method of producing oligosaccharides by the reaction between lactose and .beta.-galactosidase from aspergillus oryzae in which an concentration of lactose in an enzyme reaction mixture is between 50 and 90% (w/v) and a reaction temperature is within the range of 55.degree. C. to the temperature at which the .beta.-galactosidase in the reaction mixture is inactivated. This invention therefore makes it possible to obtain the sugar mixture containing high-purity oligosaccharides, small amounts of unreacted lactose and by produced monosaccharides.
REFERENCES:
patent: 3592739 (1971-07-01), Sternberg
patent: 3919049 (1975-11-01), Kiuchi et al.
patent: 4435389 (1984-03-01), Mutai et al.
Chemical Abstracts, Nakano et al., Nov. 23, 1987, p. 365, #193924(p).
M. Dixon et al.: "Enzymes", 2nd Edition, 1971, pp. 36,37, 144-151, 466, 467, Longman, London, GB pp. 27, 148, 467.
Kan Tatsuhiko
Kobayashi Yo-ichi
Griffin Ronald W.
Kabushiki Kaisha Yakul't Honsha
Webber Pamela S.
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