Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1980-06-05
1982-09-21
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
426 11, 435162, 435178, 435182, C12P 706, C12N 1110, C12N 1104
Patent
active
043507654
ABSTRACT:
Ethanol is produced in high concentration of 75 mg/ml or above such as 200 mg/ml by using an immbolized ethanol-producing microorganism to convert sugar to ethanol in a nutrient culture broth containing not more than 100 mg/ml sugar until the concentration of sugar is not more than 20% of its initial concentration, then adding a fresh culture broth containing not less than 100 mg/ml sugar and converting sugar to ethanol with the immobilized microorganism to produce the desired high concentration of ethanol. The microorganism is preferably immobilized in a sulfated polysaccharide gel as a dense layer near the surface of the gel.
REFERENCES:
patent: 1974937 (1934-09-01), White
patent: 4138292 (1979-02-01), Chibata et al.
Kierstan et al., The Immobilization of Microbial Cells, Subcellular Organelles, and Enzymes in Calcium Alginate Gels, Biotech. & Bioeng., vol. XIX, 1977 (pp. 387-397).
Cysemski et al., Rapid Ethanol Fermentations Using Vacuum and Cell Recycle, Biotech. & Bioeng., vol. XIX, 1977, (pp. 1125-1143).
Chibata et al., Transformations of Organic Compounds by Immobilized Microbial Cells, Advances in Applied Microbiology, vol. XXII, 1977 (pp. 1-27).
Chibata Ichiro
Kato Jyoji
Wada Mitsuru
Naff David M.
Tanabe Seiyaku Co. Ltd.
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