Method for producing amide compounds using a nitrile hydratase f

Details Method for producing amide compounds using a nitrile hydratase f Method for producing amide compounds using a nitrile hydratase f

1999-02-10

2000-11-28

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing nitrogen-containing organic compound

435183, C12P 1302, C12N 900

Patent

active

061534153

ABSTRACT:
A process for the bioconversion of a nitrile to its corresponding amide product, particularly acrylonitrile to acrylamide which is used for forming polymers. The process uses a thermophilic bacterium having a nitrile hydratase activity that is constitutively expressed, activated by cobalt ions, stable at 60.degree. C., and is most active between 20.degree. C. to 70.degree. C. with optimum activity at 55.degree. C. Alternatively, the process uses the enzyme extracted from the thermophilic bacterium to convert a nitrile to its amide product. The genes encoding nitrile hydratase and amidase are described in which the former is useful for the conversion of an nitrile to its amide and the later is useful for the conversion of an amide to its acid.

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