Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2006-04-03
2008-10-14
Horlick, Kenneth R. (Department: 1637)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
Reexamination Certificate
active
07435811
ABSTRACT:
Provided is a method of removing protein while not removing nucleic acids from a biological sample containing protein, the method including: adding a compound of formula I below and a protein nucleating agent to the biological sample containing protein:where at least two of R1, R2, and R3substituents are substituted or unsubstituted C1-C6 alkyl groups and the other substituent is a hydrogen atom or a substituted or unsubstituted C1-C6 alkyl group, a is an integer of 1 to 6, and b is 0 or 1, wherein b is 0 when a is not 1; treating the resultant mixture with a hydrophobic surface material in order to obtain a protein-free mixture; and separating the protein-free mixture from the hydrophobic surface material to which the protein is bound. By using the method, the protein can be selectively, effectively removed from the biological sample containing the protein while a nucleic acid is maintained in the sample.
REFERENCES:
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patent: 6921537 (2005-07-01), Zlotnick
Alan D. Antoine, Chairperson, Department of Biochemistry and Microbiology, Rutgers University, 76 Lipman Drive, New Brunswick, New Protein Purification: Precipitation, course numbers 115:412/508, Proteins and Enzymes, spring 2004, also found at: www.cook.rutgers.edu/˜dbm/precipitations04.pdf.
Hengen, Methods and reagents—Optimizing multiplex and LAoPCR with betaine, TIBS 22—Jun. 1997, pp. 225-226.
Schneider, W. C., “Phosphorus Compounds In Animal Tissues,”J. Biol. Chem.(1945) 161: 293-303.
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Han Ki-woong
Lee Myo-yong
Cantor & Colburn LLP
Horlick Kenneth R.
Samsung Electronics Co,. Ltd.
Staples Mark
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