Method for maintaining immobilized glucose isomerase activity du

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435179, 435180, 435234, C12P 1924, C12N 1112, C12N 1108, C12N 992

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active

051770059

ABSTRACT:
This invention provides a method for the maintenance of continuous activity of an immobilized enzyme reaction by initially underloading an adsorbent with a desired enzyme, monitoring the activity of the enzyme and periodically adding fresh enzyme to maintain a constant, continuous level of activity until the maximum carrying capacity of the support is reached. The method is preferably carried out when continuously isomerizing glucose to fructose with glucose isomerase adsorbed to a weakly basic anion exchange resin.

REFERENCES:
patent: 3788945 (1974-01-01), Thompson et al.
patent: 3909354 (1975-09-01), Thompson et al.
patent: 3960663 (1976-06-01), Tamura et al.
patent: 4110164 (1978-08-01), Sutthoff et al.
patent: 4355117 (1982-10-01), Antrim et al.
patent: 4411996 (1983-10-01), Lloyd
Immobilized Enyzmes--Modern State & Prospects Edited by I. V. Berezin et al., vol. 1, 1974, pp. 133-135-In Russian.
Enzyme Activity Maintenance In Packed Bed Reactors Via Continuous Enzyme Addition; F. H. Verhoff et al.; Biotechnology & Bioengineering, vol. XXIII, pp. 41-60, 1981.

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