Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-01-23
2007-01-23
Martinell, James (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200
Reexamination Certificate
active
10169371
ABSTRACT:
The invention relates to a method for generating, and optionally detecting, an oligonucleotide, comprising at least the steps of: a) providing a first dsDNA; b) ligating the first dsDNA to a second dsDNA, in which said second dsDNA sequence comprises within its sequence at least one recognition site for an IIS restriction endonuclease; so as to provide a ligated dsDNA; d) restricting the ligated dsDNA with the at least one restriction endonuclease of the IIS type so as to obtain at least a first and a second IIS-restricted dsDNA; and optionally comprising the further step of: e) detecting the first and/or second IIS-restricted dsDNA obtained in the restriction step d). Preferably, said method comprises the further step of: c) amplifying the ligated dsDNA obtained in the ligating step b) prior to the restriction step d). The detection step e) preferably comprises the steps of: 1) generating at least one ssDNA from either the first or the second IIS-restricted dsDNA obtained in the restriction step d); 2) detecting the at least one ssDNA generated from either the first or the second IIS-restricted dsDNA; and is more preferably based upon the molecular mass of, and/or the nucleotide sequence of, the fragment(s) obtained in the restriction step d), such as a mass spectroscopy technique, in particular of MALDI-TOF; and/or a chromatography technique, such as high pressure liquid chromatography (HPLC); or a suitable combination thereof, such as Gas Chromatography-Mass Spectroscopy (GC-MS).
REFERENCES:
patent: 5710000 (1998-01-01), Sapolsky et al.
patent: 0 534 858 (1993-03-01), None
patent: WO 94/16090 (1994-07-01), None
patent: WO 97/33000 (1997-09-01), None
patent: WO 98/08981 (1998-03-01), None
patent: WO 00/15852 (2000-03-01), None
patent: WO 00/34518 (2000-06-01), None
patent: WO 00/53806 (2000-09-01), None
Sears et al, Nucleic Acids Res. 24 (18), 3590 (1996).
Wong et al “General method for HPLC purification and sequencing of selected dsDNA gene fragments from complex PCRs generated during gene expression profiling” BIOTECHNIQUES, vol. 28, No. 4, pp. 776-783, 2000.
Laken et al, “Genotyping by mass spectrometric analysis of short DNA fragments”, Nature Biotechnology, vol. 16, pp. 1352-1356, Dec. 1998.
Szybalski et al, “Class IIS restriction enzymes- a review” GENE, vol. 100, pp. 13-26, 1991.
Wu et al, “Time-of-flight mass spectrometry of underivatized single-stranded DNA oligomers by matrix-assisted laser desorption”, Anal. Chem., vol. 66, pp. 1637-1645, 1994.
Vos et al, “AFLP: a new technique for DNA fingerprinting” Nucleic Acids Research, vol. 23, No. 21, pp. 4407-4414, 1995.
Douglas Smith, “Ligation-mediated PCR of restriction fragments from large DNA molecules”, PCR Methods and Applications, vol. 2, No. 1, pp. 21-27, Aug. 1992.
Douglas Jones, “An iterative and regenerative method for DNA sequencing” BIOTECHNIQUES, vol. 22, No. 5, pp. 938-946, 1997.
Heijnen Leo
Hogers René Cornelis Josephus
Van Eijk Michael Josephus Theresia
Browdy & Neimark PLLC
Keygene N.V.
Martinell James
LandOfFree
Method for generating oligonucleotides, in particular for... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for generating oligonucleotides, in particular for..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for generating oligonucleotides, in particular for... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3804501