Method for facilitating the introduction of material into cells

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Mutation employing radiation or electricity

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435 6, 4351735, 4351736, 4351737, 435325, 435358, 435455, 435461, 435470, 435471, C12N 1500, C12N 1585, C12Q 168

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active

060603151

ABSTRACT:
The present invention is a method for creating a localized disruption within a boundary of a cell or structure by exposing a boundary of a cell or structure to a set of energetically charged particles while regulating the energy of the charged particles so that the charged particles have an amount of kinetic energy sufficient to create a localized disruption within an area of the boundary of the cell or structure, then upon creation of the localized disruption, the amount of kinetic energy decreases to an amount insufficient to create further damage within the cell or structure beyond the boundary. The present invention is also a method for facilitating the introduction of a material into a cell or structure using the same methodology then further exciting the area of the boundary of the cell or structure where the localized disruption was created so to create a localized temporary opening within the boundary then further introducing the material through the temporary opening into the cell or structure.

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