Method for extra-cellular expression of protein

Details Method for extra-cellular expression of protein Method for extra-cellular expression of protein

1994-07-15

1997-08-19

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 694, 435 6951, 435 6952, 435 696, 435 698, 435226, 435228, 435231, C12P 2100, C12N 964, C12N 980, C12N 986

Patent

active

056587554

ABSTRACT:
Heterologous extra-cellular expression of recombinant proteins in soluble functional form is desirable because of the ease associated with purification of the secreted proteins and avoidance of the need for cell extraction and protein refolding procedures. The present invention provides DNA sequences of the naturally-occurring phthalyl amidase gene isolated from Xanthobacter agilis that control transcription, translation, and extra-cellular secretion of proteins in Streptomyces lividans. These DNA sequences can be used in a method for extra-cellular expression of a wide variety of proteins in soluble functional form.

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patent: 5032510 (1991-07-01), Kovacevic et al.
patent: 5200327 (1993-04-01), Garvin et al.
patent: 5451522 (1995-09-01), Queener et al.
Kuhstoss et al. (1991), Gene 103:97-99.
Smith, Gale E., et al., Molecular and Cellular Biology, vol. 3, No. 12, pp. 2156-2165, Dec. 1983.

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