Method for eliminating glucose dependent Schiff base effect from

Chemistry: analytical and immunological testing – Hemoglobin – myoglobin – or occult blood – Glycosylated hemoglobin

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210656, 422 61, 436 95, G01N 3366, G01N 3372

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active

044093357

ABSTRACT:
An ion exchange method for the separation of hemoglobin A.sub.1 from its Schiff base precursors and from nonglycosylated hemoglobin in a sample of human blood. The known method of lysing the sample, using it to impregnate a weak cation exchange resin, eluting out the glycosylated components with a buffer solution containing from about 0.6 M to about 0.11 M alkali metal ion dissolved therein, and recovering the eluate, is modified by the inclusion of a dihydroxyboryl compound in either the hemolysate, the elution buffer, or both.

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