Method for determining if tissue is malignant as opposed to non-

Details Method for determining if tissue is malignant as opposed to non- Method for determining if tissue is malignant as opposed to non-

1993-08-27

1995-11-21

Cohen, Lee S.

Surgery

Truss

Pad

128633, 606 3, 606 10, 606 13, 607 88, 607 89, A61B 600

Patent

active

054677670

ABSTRACT:
A method for determining if tissue is malignant as opposed to non-malignant (i.e., benign tumor tissue, benign tissue, or normal tissue). In one embodiment, the method comprises irradiating a human breast tissue sample with light at a wavelength of about 310 nm and measuring the time-resolved fluorescence emitted therefrom at about 340 nm. The time-resolved fluorescence profile is then compared to similar profiles obtained from known malignant and non-malignant human breast tissues. By fitting the profiles to the formula I(t)=A.sub.1 e.sup.(-t/.tau. 1.sup.) +A.sub.2 e.sup.(-t/.tau. 2.sup.), one can quantify the differences between tissues of various conditions. For example, non-malignant human breast tissues exhibit a slow component (.tau..sub.2) which is less than 1.6 ns whereas malignant human breast tissues exhibit a slow component (.tau..sub.2) which is greater than 1.6 ns. In addition, non-malignant human breast tissues exhibit a ratio of fast to slow amplitudes (A.sub.1 /A.sub.2) which is greater than 0.85 whereas malignant human breast tissues exhibit a ratio of fast to slow amplitudes (A.sub.1 /A.sub.2) which is less than 0.6. This technique can be used with different excitation and/or emission wavelengths, and can be applied to the detection of malignancies (or other abnormal states) in tissues other than human breast tissue.

REFERENCES:
patent: 3941121 (1976-03-01), Olinger et al.
patent: 3961621 (1976-06-01), Northeved
patent: 4311138 (1982-01-01), Sugarman
patent: 4410020 (1983-10-01), Lorenz
patent: 4502487 (1985-03-01), DuBrucq et al.
patent: 4532935 (1985-08-01), Wang
patent: 4566438 (1986-01-01), Liese et al.
patent: 4622974 (1986-11-01), Coleman et al.
patent: 4930516 (1990-06-01), Alfano et al.
patent: 4957114 (1990-09-01), Zeng et al.
patent: 5042494 (1991-08-01), Alfano
patent: 5131398 (1992-07-01), Alfano et al.
patent: 5280788 (1994-01-01), Janes et al.
D. B. Tata et al., "Fluorescence Polarization Spectroscopy and Time-Resolved Fluorescence Kinetics of Native Cancerous and Normal Rat Kidney Tissue", Biophys. J., vol. 50, pp. 463-469, Sep. 1986.
Edholm et al., "Tissue Identification During Needle Puncture By Reflection Spectrophotometry", Med. & Biol. Engng., vol. 6, pp. 409-413, 1968.

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