Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
1998-11-12
2002-05-21
Jones, W. Gary (Department: 1655)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C536S024300, C536S024310, C536S023100, C536S025400
Reexamination Certificate
active
06391546
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a method for detecting a target nucleotide sequence using a complementary nucleotide sequence.
2. Background Art
A number of methods for detecting a target deoxyribonucleic acid (DNA) using a DNA that is complementary to the DNA sequence (“complementary DNA”) are well known. A typical example is the Southern blotting method to identify a specified DNA. Plaque hybridization and colony hybridization used in DNA cloning are also well known technique. The target DNA has first to be separated into a single strand as these methods are based on the fact that a target DNA hybridizes specifically to its complementary DNA.
However, with the exception of the Southern blotting method in which it is immobilized on a membrane, a single-strand DNA becomes spherical and such spherical DNA can not hybridize with the complementary DNA. Furthermore, it is necessary to heat a double-stranded DNA in order to convert it to a single-strand DNA. However such treatments adversely affect the target DNA.
SUMMARY OF THE INVENTION
The inventors found that the sensitivity of detecting a target nucleotide sequence using a complementary nucleotide sequence is remarkably improved by converting the target nucleotide sequence into a partially double-stranded nucleotide sequence. The present invention is based on this finding.
An object of the present invention is to provide a method of detecting a target nucleotide sequence using a complementary nucleotide sequence that has an excellent sensitivity of detection.
Another object of the present invention is to provide a method of producing a partially double-stranded nucleotide sequence that is used for the method of detecting the target nucleotide sequence.
The method of detecting a target nucleotide sequence according to the present invention comprises the steps of converting a target nucleotide sequence into a partially double-stranded nucleotide sequence and detecting the partially double-stranded nucleotide sequence using a complementary nucleotide sequence to the target nucleotide sequence (“complementary nucleotide sequence”).
REFERENCES:
patent: 5030557 (1991-07-01), Hogan et al.
patent: 5464743 (1995-11-01), Weisburg et al.
patent: 5474895 (1995-12-01), Ishii et al.
patent: 5485277 (1996-01-01), Foster
patent: 5637685 (1997-06-01), Soares et al.
patent: 5695926 (1997-12-01), Cros et al.
patent: 5753439 (1998-05-01), Smith et al.
patent: 6007987 (1999-12-01), Canto et al.
patent: 0 622 464 (1994-11-01), None
patent: 6-30797 (1984-02-01), None
patent: 4-320700 (1992-11-01), None
patent: 5-503215 (1993-06-01), None
patent: 5-252998 (1993-10-01), None
patent: 7-174693 (1995-07-01), None
patent: WO 91/13174 (1991-09-01), None
Karube Isao
Nagata Ryohei
Sawata Shinya
Jones W. Gary
Karube Isao
Oblon & Spivak, McClelland, Maier & Neustadt P.C.
Taylor Janell E.
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