Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1994-01-31
1995-08-22
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 14, 435 29, 435 39, 435243, 4352536, 435261, 435291, 435299, 435310, 435805, 435810, 435882, C12Q 104, C12Q 154, C12N 100
Patent
active
054439639
ABSTRACT:
The present invention exploits the herein first reported, empirical observation that even though staphylococci produce the enzyme, beta-glucosidase, this genus of bacteria is not able to produce a metabolite that will enzymatically react with 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside, a substrate commonly used to detect the presence of beta-glucosidase produced by other bacteria. In view of this unexpected observation, one embodiment of the present invention includes a selective medium containing inhibitors to enhance staphylococci growth as well as a first glucopyranoside substrate, such as 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside, and a second phosphatase substrate, such as 6-chloro-3-indolylphosphate or 5-bromo-6-chloro-3-indolylphosphate. In this embodiment, staphylococci in a sample will produce metabolites that will react with the phosphate substrate in the medium to produce colonies having red to red-violet color while other bacteria in the sample will produce beta-glucosidase that will react with the glucopyranosidase substrate in the medium and produce colonies having a blue color. In a particularly preferred embodiment of this invention, a thin film culture plate device, such as a PETRIFILM culture plate device, is prepared using a dry culture medium containing selected inhibitors, a first glucopyranoside substrate and a second phosphate substrate. When the thin film culture plate device is inoculated with a sample and then incubated for a sufficient period of time, staphylococci in the sample will produce colonies on the thin film device having a red color and other bacteria in the sample will produce colonies on the device having a blue color.
REFERENCES:
patent: Re24906 (1960-12-01), Ulrich
patent: 4476226 (1984-10-01), Hansen et al.
patent: 4565783 (1986-01-01), Hansen et al.
patent: 4895745 (1990-01-01), Vesley et al.
patent: 5089413 (1992-02-01), Nelson et al.
patent: 5210022 (1993-05-01), Roth et al.
patent: 5232838 (1993-08-01), Nelson et al.
Arakawa et al, Analitical Biochemistry, vol. 199, pp. 238-242, 1991.
Sigma, `Biochemical Organic Compounds for Research and Diagnostic Reagents, (Catalog), p. 182, 1993.
U.S. patent application Ser. No. 07/804,296, Crandall et al., "Nonionic, pH-nuetral Pressure Sensitive Adhesive", filed Dec. 9, 1991.
Busse Paul W.
Griswold Gary L.
Kirn Walter N.
Minnesota Mining and Manufacturing Company
Mohamed Abdel A.
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