Method for detecting a target nucleic acid by using an interacti

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435810, 436501, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, C12Q 168

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057053464

ABSTRACT:
A method for detecting a target nucleic acid comprises the steps of reacting a sample with a probe in the presence of two or more kinds of reagents capable of being made an irreversible change capable of being detected and accumulating by an interaction through a double helix structure under a condition enabling the replication of the formation and dissociation of a hybrid composed of the target nucleic acid in the sample and the probe, accumulating the irreversible change caused by the interaction of the reagents, and then detecting the accumulated change.

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Strobel et al. Preparation and Characterization of Spin-Labeled Oligonucleotides for DNA Hybridization, Bioconj. Chem (1991) 2:89-95.
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