Chemistry: analytical and immunological testing – Lipids – triglycerides – cholesterol – or lipoproteins
Patent
1994-09-06
1996-03-05
Wityshyn, Michael G.
Chemistry: analytical and immunological testing
Lipids, triglycerides, cholesterol, or lipoproteins
436 63, 436811, G01N 3392
Patent
active
054967350
ABSTRACT:
A new method for determining and reporting lipid fatty acids compositions and its use in diagnosing diabetes is disclosed. Adding internal standards, which are true analogs of the fatty acids to be analyzed, to blood serum or plasma samples before performing a gas chromatographic analysis of the fatty acids allows the results of the analysis to be reported in absolute terms as, for example, mg/L, instead of in relative percentages. Reporting the results as absolute concentrations reveals statistically significant differences in levels of various lipid fatty acids between persons having and not having diabetes.
REFERENCES:
patent: 3751381 (1973-08-01), Megraw
patent: 3764556 (1973-10-01), Kuchmak et al.
patent: 3955925 (1976-05-01), Proksch et al.
patent: 4239649 (1980-12-01), Gindler et al.
patent: 4290774 (1981-09-01), Girgis et al.
patent: 4868129 (1989-09-01), Deeq et al.
patent: 5075101 (1991-12-01), Siguel
patent: 5262406 (1994-11-01), Vitale
H. A. Schwertner et al., Comparison of Lipid Fatty Acids on a Concentration Basis vs Weight Basis in Patients With and Without Coronary Artery Disease or Diabetes, Clinical Chemistry, vol. 29, No. 4, 1993, pp. 659-663.
L. Stokes et al., Gas-Liquid Chromatographic Analysis of Cellular Fatty Acids for Identification of Gram-Negative Anaerobic Bacilli, Journal of Clinical Microbiology, vol. 29, No. 11, Nov. 1991, pp. 2626-2638.
P. Kotilainen, et al., Application of Gas-Liquid Chromatiographic Analysis of Cellular Fatty Acids for Species Identification and Typing of Coagulase-Negative Staphylococci, Journal of Clinical Microbiology, Feb. 1991, pp. 315-322.
J. T. Salonen, Serum Fatty Acids, Apolipoproteins, Selenium and Vitamin Antioxidants and the Risk of Death From Coronary Artery Disease, The American Journal of Cardiology, Aug. 1, 1985, pp. 226-231.
T. A. Miettinen et al., Fatty-Acid Composition of Serum Lipids Predicts Myocardial Infarction, Clinical Research, Oct. 1982, pp. 993-995.
H. A. Schwertner et al., Increased Concentrations of Free Fatty Acids and Glycerol and Altered Creatine Kinase MM Isoenzyme Patterns in Certain Diabetic Patients, Clinical Chemistry, vol. 25, No. 4, 1979, pp. 520-522.
Shimomura et al., "Quantitative determination of the fatty acid . . . " J. Chromatog. (1986, Nov. 28) 383(1) 9-17.
Tilvis et al. "Fatty-acid Compositions of Serum . . . " J. Clin. Endocrinol Metab. 61(4). 1985. 741-745.
Tsuchiya et al. "Simultaneous separations and sensitive . . . " J. Chromatog. (1984 Jul. 13) 309(1) 43-52.
Freed Rachel Heather
Kundert Thomas L.
Sinder Fredric L.
The United States of America as represented by the Secretary of
Wityshyn Michael G.
LandOfFree
Method for concentration based analysis of lipid fatty acids and does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for concentration based analysis of lipid fatty acids and, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for concentration based analysis of lipid fatty acids and will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1412528