Method, device and kit for measurement of tissue plasminogen act

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 74, 435 13, 435 23, 436518, 436548, 935110, G01N 33543, G01N 33577

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active

051027879

ABSTRACT:
A true activity of tissue plasminogen activator is determined by reacting a solid phase having bound thereto anti tissue plasminogen activator antibody IgG fraction having affinity to a site other than the active site of tissue plasminogen activator with tissue plasminogen activator in a sample and then measuring an activity of the reaction product, preferably in terms of an amount of a substance released from a chromogenic peptide substrate. This measurement enables to use of whole blood or diluted blood as the sample and achieves high sensitivity and high accuracy in a short period of time.

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Norrman et al., Chem. Abstr., 108, 110510x, 1988.
Angles-Cano et al., Blood, 66, 913-920, 1985.
Holovet et al., Eur. Journ. Biochem., 158, 173-177, 1986.
Van Zonneveld et al., Thrombosis and Haemeostasis, 57, 82-86, 1987.

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