Method and apparatus for the rapid disruption of cells or...

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

Reexamination Certificate

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C435S288500, C435S288700, C435S306100

Reexamination Certificate

active

07855069

ABSTRACT:
A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.

REFERENCES:
patent: 5234809 (1993-08-01), Boom et al.
patent: 5403710 (1995-04-01), Weisburg et al.
patent: 5705628 (1998-01-01), Hawkins
patent: 5863502 (1999-01-01), Southgate et al.
patent: 6043080 (2000-03-01), Lipshutz et al.
patent: 6156576 (2000-12-01), Allbritton et al.
patent: 6335201 (2002-01-01), Allbritton et al.
patent: 6613525 (2003-09-01), Nelson et al.
patent: 6685730 (2004-02-01), West et al.
patent: 6783736 (2004-08-01), Taylor et al.
patent: 6914137 (2005-07-01), Baker
patent: 7192560 (2007-03-01), Parthasarathy et al.
patent: 2003/0095897 (2003-05-01), Grate et al.
patent: 2003/0096429 (2003-05-01), Baeumner et al.
patent: 2006/0094051 (2006-05-01), Lee et al.
patent: 2006/0110725 (2006-05-01), Lee et al.
patent: 2006/0188876 (2006-08-01), Kilaas et al.
patent: 1655366 (2006-05-01), None
patent: 2000-029541 (2000-01-01), None
patent: 2004-049105 (2004-02-01), None
patent: 9945372 (1999-09-01), None
patent: 0073412 (2000-12-01), None
Deggerdal, Arne and Larsen, Frank, “Rapid Isolation of PCR-Ready DNA from Blood, Bone Marrow and Cultured Cells, Based on Paramagnetic Beads,”Bio Techniques(1997) 22(3): 554-557.
Li, Huaina et al, “Spatial Control of Cellular Measurements with the Laser Micropipet,”Anal. Chem. (2001) 73: 4625-4631.
Rudi, K. et al., “Rapid, Universal Method of Isolate PCR-Ready DNA Using Magnetic Beads,”BioTechniques, (1997) 22: 506-511.
Taylor, Michael T. et al., “Lysing Bacterial Spores by Sonication through a Flexible Interface in a Microfluidic System,”Anal. Chem. (2001) 73: 492-496.
European Search Report; Application No. EP 05 02 2672; Date; Jun. 14, 2006.
“Self Contained, Fully Integrated Biochip for Sample Preparation, Polymerase Chain Reaction Amplification, and DNA Microarray Detection”; Authors: Robin Hui Liu, Jianing Yang, Ralf Lenigk, Justin Bonanno, and Piotr Grodzinski; Analytical Chemistry, vol. 76, No. 7, Apr. 1, 2004; 1824-1831 XP-001196720.
“Recent Developments of Magnetic Beads for Use in Nucleic Acid Purification”; Authors: Peter R. Levison, Stephen E. Bader, Jon Dennis, Prit Hathi, Martin J. Davies, Ian J. Bruce, Dieter Schimkat; Journal of Chromatography A. 816 (1998) 107-111.
“Quantitative Cell Lysis of Indigenous Microorganisms and Rapid Extraction of Microbial DNA From Sediment”; Authors: Margaret I. More, James B. Herrick, Margarida C. Silva, William C. Ghiorse and Eugene L. Madsen; Applied and Environmental Microbiology, May 1994, p. 1572-1580; XP 000574303.
“Laser Based Disruption ofBacillusSpores on a Microchip”; Authors: Oliver Hofmann, Kirk Murray, Alan-Shaun Wilkinson, Timothy Cox, Andreas Manz; 8th International Conference on Miniaturized Systems for chemistry and Life Sciences; Sep. 26-30, 2004, XP009066976.
Elgort, M.G., et al.; “Extraction and Amplification of Genomic DNA from Human Blood on Nanoporous Aluminum Oxide Membranes”; Clinical Chemistry; vol. 50, No. 10; pp. 1817-1819; 2004.
Li, H., et al.; “Selective genotyping of individual cells by capillary polymerase chain reaction”; Electrophoresis; vol. 23; pp. 3372-3380; 2002.
Tian, H., et al.; “Evaluation of Silica Resins for Direct and Efficient Extraction of DNA from Complex Biological Matrices in a Miniaturized Format”; Analytical Biochemistry; vol. 283; pp. 175-191; 2000.
Safarik, I., et al.; “Biologically Active Compounds and Xenobiotics: Magnetic Affinity Separations”; Encyclopedia of Separation Science, Academic Press; pp. 2163-2170; 2000.
Leszczynski D. et al, Laser-beam-triggered Microcavitation: a novel method for selective cell destruction, Radiation Res. 2001; 156: 399-407.

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