Method and apparatus for sterile cultivation of cells on solid s

Plant husbandry – Mushroom culture

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71 5, A01G 104

Patent

active

042043646

ABSTRACT:
The invention disclosed comprises a process and apparatus for the cultivation of cells, and having particular application to the product of mushroom spawn, in which a particulate substrate, water and, in some cases chalk, are introduced into a rotary blender and thoroughly mixed and sterilized at a temperature of about 250.degree. F. The mixture is cooled in the blender and a cell line inoculum is sterilely introduced into the mixture and thoroughly blended with the substrate. The blended inoculum and substrate are transferred in a sterile air flow from the blender to sterile containers which include a breathing strip in which the cells lines are incubated. The apparatus includes the combination of blender, transfer means and a clean room for filling the sterile containers.

REFERENCES:
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patent: 2520318 (1950-08-01), Lescarboura
patent: 2677917 (1954-05-01), Speakman
patent: 2723493 (1955-11-01), Stoller
patent: 2851821 (1958-09-01), Guichon
patent: 3177615 (1965-04-01), Rowe
patent: 3335521 (1967-08-01), Sohm
patent: 3938658 (1976-02-01), Rohde
patent: 4059919 (1977-11-01), Green
patent: 4063383 (1977-12-01), Green

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