Method and apparatus for observing a specimen using an X-ray mic

Details Method and apparatus for observing a specimen using an X-ray mic Method and apparatus for observing a specimen using an X-ray mic

1996-09-20

1998-08-04

Church, Craig E.

X-ray or gamma ray systems or devices

Specific application

Telescope or microscope

378210, G21K 700

Patent

active

057906270

ABSTRACT:
X-ray microscopy is used to observe a specimen by labelling the specimen with a molecule having a double bond, such as, for example, N-succinimidyl-4-nitrophenyl acetate or 5-(dimethylamino phenyl)-2,4-pentadienal, which bond to an amino group, and O-(4-nitrobenzyl)-N,N-diisopropyl isourea, which chemically bonds to a carbonyl group. Such labelling groups can generate fluorescence to facilitate the observation of the specimen. Observation is improved by using a monochromatic X-ray source having a photon energy lower than 2000 eV and a band width narrower than 1 eV.

REFERENCES:
patent: 5107526 (1992-04-01), Hoover

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